Registry Code |
Name |
Role |
Description |
Length (bp) |
BBa_K1819003 |
RoxA |
Protein coding |
Rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y- catalyzes oxidative C-C cleavage of
poly(cis-1,4-isoprene) |
1954 (bp) |
BBa_K1819001 |
Lcp |
Role |
Latex clearing protein (Lcp) from Streptomyces sp. strain K30
- catalyzes rubber cleavage at the
cis double bonds |
1138 |
BBa_K1819000 |
Linker-GFP |
Reporter part |
Is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site |
750 |
BBa_K1819002 |
TAT signal |
Signalling part |
Twin-arginine translocation signal |
147 |
BBa_K1819004 |
GFP_I13507 |
Composite part |
RFP regulation through promoter tetR (part R0040), dependent of tetracycline |
2499 |
BBa_K1819005 |
J23101 + linkerGFPTerm |
Characterization circuit |
Constitutive promotor from Anderson library (BBa_J23101) assembled to green fluorescent protein attached to linker |
930 |
BBa_K1819006 |
J23106 + linkerGFPTerm |
Characterization circuit |
Constitutive promoter from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker |
930 |
BBa_K1819007 |
J23117 + linkerGFPTerm |
Characterization circuit |
Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker |
930 |
BBa_K1819008 |
Pl_I13507 |
Construction promoter test |
Promoter test circuit to analyze the kill switch efficiency |
2725 |
BBa_K1819009 |
pVeg + GFP_Term |
Characterization circuit |
This device express GFP under a strong constitutive Bacillus promoter control (pVeg) (BBa_K823003) |
1123 |
BBa_S05291 |
RBS-OmpA |
Construction intermediate |
It is composed of a RBS preceding an outer membrane protein A (OmpA), that displays proteins on cell surface. |
484 |
BBa_S05307 |
GFP_P0440 |
Construction intermediate |
This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR |
1568 |
BBa_S05308 |
Linker-GFP_Term |
Construction intermediate |
It is an intermediate construction of the export test circuit fused to a terminal region |
887 |