Team:Brasil-USP/Notebook/Calendar
Notebook
Calendar
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July
Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday |
01/06
- Agarose gel (RBS_hokD) - Gel purification (RBS_hokD) - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) - Agarose gel (P0040) - Gel purification (P0040) - Ligation Pt_I13507(P0040 + I13507) - Interlab gel purification and ligation |
02/06
- Transformation Ptet_I13507 - Agarose gel (Pl_RBS, GFP_P0440) - Gel purification (Pl_RBS, GFP_P0440) - Ligation Pl_P0440 (Pl_RBS + GFP_P0440) - Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002) |
03/06
- Transformation (Pr_RBS, 2X_RBS_hokD) - EXSP digestion (P0440, R0040) - Transformation Pl_R0440 (Pl_RBS + GFP_P0440) - Inoculum (Pt_I13507) |
04/06
- Agarose gel (P0440, R0040) - Gel purification (P0440, R0040) - Ligation tet_Ptet (P0440 + R0040) - Inoculum (2X_RBS_hokD) - Miniprep (Pt_I13507) |
05/06
- Inoculum (R0080, K91403) - EXSP digestion (tet_Term, Pt_E0840) - Miniprep (2X_RBS_hokD) |
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06/06
- NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS) - Transformation (tet_Ptet) - Agarose gel (2X_RBS_hokD) – positive results - Agarose gel (tet_Term, Pt_E0840) - EXSP digestion (Pt_E0480) - Inoculum (Pl_P0440) - NotI digestion (Pt_I13507) – positive results |
07/06
- Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840) - EXSP digestion (2X_RBS_hokD, B0015) - Ligation Term_E0840 (Pt_E0840 + tet_Term) - Inoculum (tet_Ptet) - Transformation (Term_E0840) - Miniprep (Pl_P0440) - Not digestion (Pl_P0440) – positive results - EXSP digestion (Pl_P0440, Pt_I13507) - Inoculum (GFP_P0440) |
08/06
- Agarose gel (2X_RBS_hokD, B0015) – B0015 didn’t digest - Gel purification (2X_RBS_hokD) - EXSP digestion (B0015) - Miniprep (tet_Ptet) - NotI digestion (tet_Ptet) - Agarose gel (tet_Ptet, B0015) – positive results - Gel digestion (B0015) - Ligation hokD_Term (2X_RBS_hokD + B0015) - Ligation Pl_I13507 (Pl_P0440 + Pt_I13507) - Miniprep (GFP_P0440) - EXSP digestion (GFP_P0440) |
09/06
- Agarose gel and gel purification (GFP_P0440) - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507) - Transformation (Pl_I13507) |
10/06
- Transformation (hokD_Term) - Inoculum (Pl_I13507) - Transformation (GFP_I13507) | 11/06
- Miniprep (Pl_I13507) |
12/06 |
13/06
- Inoculum (GFP_I13507) - NotI digestion (Pl_I13507) - Agarose gel (Pl_I13507) – negative results |
14/06
- Miniprep (GFP_I13507) - NotI digestion (GFP_I13507) – negative results - EXSP digestion (Pl_P0440, Pt_I13507) - Inoculum (GFP_P0440, Pt_I13507) | 15/06
- Agarose gel (Pl_P0440, Pt_I13507) - Gel purification (Pl_P0440, Pt_I13507) - Ligation Pl_I13507 (Pl_P0440 + Pt_I13507) - Miniprep (GFP_P0440, Pt_I13507) - EXSP digestion (GFP_P0440, Pt_I13507) |
16/06
- Agarose gel and gel purification (GFP_P0440, Pt_I13507) - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507) - Transformation (Pl_I13507) | 17/06
- Transformation (GFP_I13507) - Inoculum (Pt_Term, Pl_I1350) |
18/06
- Miniprep (Pt_Term, Pl_I13507) - NotI digestion (Pt_Term, Pl_I13507) - Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term | 19/06 |
20/06
| 21/06
- Inoculum (GFP_I13507, Pl_I13507) - New interlab transformations |
22/06
- Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - EXSP digestion (Pr_RBS, R0080) - Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS) - Gel purification (Pr_RBS) - Inoculum (GFP_I13507, Pl_I13507) - Interlab inocula | 23/06
- Agarose gel (R0080) - Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507 - EXSP digestion (GFP_I13507) - Interlab miniprep and restriction analysis |
24/06
- Miniprep (B0015, Pt_P0440) - EXSP digestion (2X_RBS_hokD, B0015) - Transformation (Pa_RBS) - Agarose gel of restriction analysis: devices confirmed | 25/06
- agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest |
26/06
- Agarose gel and gel purification (GFP_I13507) - Ligation Pr_I13507 (Pr_RBS + GFP_I13507) |
27/06
- EXSP digestion (Pl_RBS, Pt_Term) - Transformation (Pr_I13507, Pa_RBS) |
28/06
- Agarose gel (Pl_RBS, Pt_Term) ligation Pr_I13507(Pr_RBS + Pt_Term) |
29/06
- EXSP digestion (B0015, 2X_RBS_hokD) - agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest - EXSP digestion overnight (2X_RBS_hokD) - transformation (Pr_Term, Pl_RBS) |
30/06
- inoculum (Pl_RBS, Pa_RBS) - agarose gel (B0015, 2X_RBS_hokD) - Digestion worked! - ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD) |
31/06 |
August
Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday | |
01/08 | 02/08 | 03/08
- EXSP digestion (R0010, R0080, B0034, Pt_I13507) - NotI digestion (Pl_I13507) - Ligation Pa_RBS (R0080 + B0034) and Pl_RBS (R0010 + B0034) |
04/08
- NotI digestion (Pr_I13507) - Agarose gel (Pr_I13507) - Positive results!! - EXSP digestion (Pt_I13507) - Transformation (Pl_RBS and Pa_RBS) |
05/08
- agarose gel (Pl_I13507, Pt_I13507)- negative results for Pl_13507 :( - Agarose gel and gel purification (Pt_I13507) - ligation Pl_I13507 (Pl_P0440 + Pt_I13507) - inoculum (Pl_RBS, Pa_RBS) |
06/08
- Transformation (Pl_I13507, Pr_I13507) - miniprep (Pl_RBS, Pa_RBS) - NotI digestion (Pl_RBS, Pa_RBS) |
07/08
- Agarose gel (Pl_RBS, Pa_RBS) - Only Pa_RBS was positive - EXSP digestion (Pa_RBS, Pt_Term) - Ligation Pa_Term(Pa_RBS + Pt_Term) Pa_I13507 (Pa_RBS + GFP_I13507) |
08/08
- Ligation (2X_RBS_hokD + Term) - Transformation (Pa_I13507, Pa_Term) |
09/06
- Inoculum (Pa_I13507,Pl_I13507,Pa_Term) |
10/08
- miniprep (Pa_I13507, Pl_I13507, Pa_Term) - NotI digestion (Pa_I13507, Pa_Term) - Agarose gel (Pa_I13507, Pl_I13507, Pa_Term, Pl_RBS) - Positive results for Pa_I13507 and Pa_Term - Pre-inoculum of interlab study devices, BBa_K876004, and controls | 11/08
- Vector exchange digestion (Pr_RBS, GFP_I13507, GFP_P0440, Tet_Term) - Ligation with pSB1C3 (Pr_RBS, GFP_I13507, GFP_P0440, Tet_Term) - EXSP digestion (Pl_RBS, GFP_I13507, Pt_Term) - ligation (Pl_I13507, Pl_Term) - Transformation OmpA - Sample preparation of Interlab Study devices and controls (plate reader) - End-point measurement of Interlab Study devices and controls - Sample preparation of BBa_K876004 part (plate reader) - Kinetic measurement of BBa_K876004 (14 hours). |
12/08
- Transformation (Pr_RBS, GFP_I13507, GFP_P0440, Tet_Term) - Inoculum OmpA - Pre-inoculum of interlab study devices, BBa_K876004, and controls | 13/08
- Inoculum (Pr_RBS, GFP_I13507, GFP_P0440, Tet_Term) - miniprep OmpA - EXSP digestion (B0034, OmpA) - agarose gel (B0034, OmpA) - Ligation RBS_OmpA (RBS + OmpA) - Sample preparation of Interlab Study devices and controls (flow cytometry) - Measurement of Interlab Study devices and controls - Sample preparation of BBa_K876004 part (flow cytometry) - Flow cytometry measurement of BBa_K876004 (14 hours). |
14/08
- miniprep (Pr_RBS, GFP_P0440(C3), Pl_I13507) - Positive results for Pl_I13507 - and GFP_P0440 - Transformation RBS_OmpA | 15/08
- Inoculum (RBS_OmpA, GFP_I13507(C3) |
16/06
- Agarose gel and gel purification (GFP_P0440, Pt_I13507) - Ligation GFP_I13507 (GFP_P0440 + Pt_I13507) - Transformation (Pl_I13507) |
17/06
- Transformation (GFP_I13507) - Inoculum (Pt_Term, Pl_I1350) |
18/06
- Miniprep (Pt_Term, Pl_I13507) - NotI digestion (Pt_Term, Pl_I13507) - Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term | 19/06 | 20/06
| 21/06
- Inoculum (GFP_I13507, Pl_I13507) - New interlab transformations |
22/06
- Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - EXSP digestion (Pr_RBS, R0080) - Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS) - Gel purification (Pr_RBS) - Inoculum (GFP_I13507, Pl_I13507) - Interlab inocula | 23/06
- Agarose gel (R0080) - Miniprep (GFP_I13507, Pl_I13507) - NotI digestion (GFP_I13507, Pl_I13507) - Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507 - EXSP digestion (GFP_I13507) - Interlab miniprep and restriction analysis |
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24/06
- Miniprep (B0015, Pt_P0440) - EXSP digestion (2X_RBS_hokD, B0015) - Transformation (Pa_RBS) - Agarose gel of restriction analysis: devices confirmed | 25/06
- agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest |
26/06
- Agarose gel and gel purification (GFP_I13507) - Ligation Pr_I13507 (Pr_RBS + GFP_I13507) | 27/06
- EXSP digestion (Pl_RBS, Pt_Term) - Transformation (Pr_I13507, Pa_RBS) |
28/06
- Agarose gel (Pl_RBS, Pt_Term) ligation Pr_I13507(Pr_RBS + Pt_Term) |
29/06
- EXSP digestion (B0015, 2X_RBS_hokD) - agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest - EXSP digestion overnight (2X_RBS_hokD) - transformation (Pr_Term, Pl_RBS) |
30/06
- inoculum (Pl_RBS, Pa_RBS) - agarose gel (B0015, 2X_RBS_hokD) - Digestion worked! - ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD) |
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31/06 |