Team:Freiburg/Materials
Materials
A list of plasmids we used/constructed during our project can be found here.
Instruments
| Device | Name | Manufacturer | ||
|---|---|---|---|---|
| Thermocycler | Thriller | Peqlab | Erlangen | Germany | 91-D 1200 | Peqlab | Erlangen | Germany |
| Thermomixer comfort | Eppendorf | Hamburg | Germany | |
| Centrifuge | Minispin | Eppendorf | Hamburg | Germany |
| Centrifuge 5424 R | Eppendorf | Hamburg | Germany | |
| Centrifuge 5804 R | Eppendorf | Hamburg | Germany | |
| Centrifuge Avanti J-26 XP | BeckmanCoulter | Brea | USA | |
| Heater/Mixer etc | MR Hei Standard | Heidolph | Schwabach | Germany |
| Duomax 1030 | Heidolph | Schwabach | Germany | |
| Variable-speed Rock’n roller Model L-202 | Labinco BV | DG Breda | Niederlande | |
| Rotamax 120 | Heidolph | Schwabach | Germany | |
| Innova 44 | Eppendorf | Hamburg | Germany | |
| Vortex | Genius 3 | IKA | Staufen | Germany |
| Scale | LE26P-0CE | Sartorius | Göttingen | Germany |
| 440-49 N | Kern | Balingen-Frommern | Germany | |
| Vacuumpump | ME 4C NT | Vacuubrand | Wertheim | Germany |
| PCR cycler | Gene Amp PCR System 9700 | Applied Biosystems | Waltham | USA |
| Mastercycler epgradient S | Eppendorf | Hamburg | Germany | |
| Plasmagenerator | ZEPTO | Diener electronics | Ebhausen | Germany |
| Powersupply | PeqPower 250 | Peqlab | Erlangen | Germany |
| Blotter | Fastblot | Biometra | Göttingen | Germany |
| Microplate reader | Synergy H4 hybrid | BioTek | Winooski | USA |
| Multi-Imager | Fusion FX7 | Peqlab | Erlangen | Germany |
| French press | FA-078 | SLM Aminco | Urbana | USA |
| UV/Vis Spectrophotometer | NanoDrop 2000 | Peqlab | Erlangen | Germany |
| SDS-PAGE apparatus | Mighty Small II | Hoefer | Holliston | USA |
| Oven | UN 30 plus | Memmert Gmbh | Schwabach | Germany |
| Gas filter gun | Wafergard GN | Mykron Microelectronics Co | Beijing | China |
Chemicals
| Name | Manufacturer | ||
|---|---|---|---|
| Phosphoenolpyruvic acid (monopotassium salt) | Roche | Basel | Switzerland |
| Sodium creatine phosphate dibasic tetrahydrate | Sigma | St. Louis | USA |
| Magnesium acetate tetrahydrate (Mg(Oac)2) | Sigma | St. Louis | USA |
| Potassium acetate | Sigma | St. Louis | USA |
| L-Amino Acids Kit | Sigma | St. Louis | USA |
| Adenosine 3',5'-cyclic monophosphate (sodium salt) | Sigma | St. Louis | USA |
| L-Glutamic acid potassium salt monohydrate | Sigma | St. Louis | USA |
| Acetic acid | Roth | Karlsruhe | Germany |
| DL-Dithiothreitol | Promega | Madison | USA |
| ß-mercaptoethanol | Roth | Karlsruhe | Germany |
| PEG4000 | ThermoFisher | Waltham | USA |
| ATP (lithium salt) | Roche | Basel | Schweiz |
| GTP (lithium salt) | Roche | Basel | Schweiz |
| CTP (lithium salt) | Roche | Basel | Schweiz |
| UTP (lithium salt) | Roche | Basel | Schweiz |
| tRNA from E. coli MRE 600 | Roche | Basel | Schweiz |
| Folinic acid (calcium salt hydrate) | Sigma | St. Louis | USA |
| Acetoacetyl coenzyme A (sodium salt) | Santa Cruz | Dallas | USA |
| D-Luciferin | Sigma | St. Louis | USA |
| Nickel(II) sulfate hexahydrate | Sigma | St. Louis | USA |
| Ethanolamine | Sigma | St. Louis | USA |
| 1,4-Phenylene diisothiocyanate | abcr | Karlsruhe | Germany |
| 3-Aminopropyltriethoxysilane | abcr | Karlsruhe | Germany |
| (3-Glycidoxypropyl)trimethoxysilane | abcr | Karlsruhe | Germany |
| (3-Chloropropyl)trimethoxysilane | abcr | Karlsruhe | Germany |
| Na-Bis(carboxymethyl)-L-lysine | abcr | Karlsruhe | Germany |
| Biotinylated Bovine Serum Albumin | ThermoFisher | Waltham | USA |
| Bovine Serum Albumin | Roth | Karlsruhe | Germany |
| Ethanol | Roth | Karlsruhe | Germany |
| Aceton | Roth | Karlsruhe | Germany |
| 2-propanol | Roth | Karlsruhe | Germany |
| Pyridine | Sigma | St. Louis | USA |
| N,N-Dimethylformamid | Roth | Karlsruhe | Germany |
| Phosphate buffered saline | Sigma | St. Louis | USA |
Media and Buffers
| Name | Manufacturer | ||
|---|---|---|---|
| Phusion Buffer | NEB (New England Biolabs) | Ipswitch | USA |
| Phusion High-GC Buffer | NEB (New England Biolabs) | Ipswitch | USA |
| Antarctic Phosphatase Buffer | NEB (New England Biolabs) | Ipswitch | USA |
| T4 DNA Ligase Buffer | NEB (New England Biolabs) | Ipswitch | USA |
| ISO-Buffer | NEB (New England Biolabs) | Ipswitch | USA |
| CutSmart | NEB (New England Biolabs) | Ipswitch | USA |
| Buffer 2.1 | NEB (New England Biolabs) | Ipswitch | USA |
| Buffer 3.1 | NEB (New England Biolabs) | Ipswitch | USA |
| Buffer 4.1 | NEB (New England Biolabs) | Ipswitch | USA |
| FastDigest Buffer | Thermo Scientific | Waltham | USA |
| FastDigest Green Buffer | Thermo Scientific | Waltham | USA |
| LB medium | BIOSS | Freiburg | Germany |
| TAE Buffer | BIOSS | Freiburg | Germany |
| Midori Green Advance | Biozym | Hessisch Oldendorf | Germany |
| DNA Ladder | Zymo Research | Freiburg | Germany |
| HEPES | Roth | Karlsruhe | Germany |
| KOH | Roth | Karlsruhe | Germany |
| TRIS | Roth | Karlsruhe | Germany |
| Triton X-100 | Sigma | St. Louis | USA |
| Tween 20 | Roth | Karlsruhe | Germany |
| Bovine serum albumin (BSA), blot quality | Promega | Madison | USA |
Enzymes
| Name | Manufacturer | ||
|---|---|---|---|
| Phusion High-Fidelity Polymerase | NEB (New England Biolabs) | Ipswitch | USA |
| Q5 High-Fidelity Polymerase Master-Mix | NEB (New England Biolabs) | Ipswitch | USA |
| Antarctic Phosphatase | NEB (New England Biolabs) | Ipswitch | USA |
| Blunting Enzyme | NEB (New England Biolabs) | Ipswitch | USA |
| T4 DNA Ligase | NEB (New England Biolabs) | Ipswitch | USA |
| Taq DNA Ligase | NEB (New England Biolabs) | Ipswitch | USA |
| T5 Exonuclease | NEB (New England Biolabs) | Ipswitch | USA |
| EcoRI | NEB (New England Biolabs) | Ipswitch | USA |
| PstI | NEB (New England Biolabs) | Ipswitch | USA |
| PstI-HF | NEB (New England Biolabs) | Ipswitch | USA |
| BamHI | NEB (New England Biolabs) | Ipswitch | USA |
| HindIII | NEB (New England Biolabs) | Ipswitch | USA |
| AflII | NEB (New England Biolabs) | Ipswitch | USA |
| Acc65I | NEB (New England Biolabs) | Ipswitch | USA |
| SpeI | NEB (New England Biolabs) | Ipswitch | USA |
| NotI | NEB (New England Biolabs) | Ipswitch | USA |
| XbaI | NEB (New England Biolabs) | Ipswitch | USA |
| XhoI | NEB (New England Biolabs) | Ipswitch | USA |
| BamHI-FD | Thermo Scientific | Waltham | USA |
| HindIII-FD | Thermo Scientific | Waltham | USA |
| BspTI-FD | Thermo Scientific | Waltham | USA |
| Pyruvate Kinase (from rabbit muscle) | Sigma | St. Louis | USA |
| Creatine Phosphokinase (from rabbit muscle) | Sigma | St. Louis | USA |
| Streptavidin Cy5 conjugate | Thermo Scientific | Waltham | USA |
Antibodies
| Name | Manufacturer | ||
|---|---|---|---|
| anti-tetX antibody :: Goat Tetanus exotoxin polyclonal Antibody | MyBiosource | Eching | Germany |
| anti-IgG h+I antibody :: Sheep IgG h+I Antibody | MyBiosource | Eching | Germany |
| Anti-Rabbit IgG (H+L) HRP Conjugate | Promega | Madison | USA |
| Anti-Mouse IgG (H+L) HRP Conjugate | Promega | Madison | USA |
| anti-PhiYFP antibody | evrogen | Moscow | Russia |
| Rabbit anti-HCV Virus Polyclonal Antibody | Anogen | Mississauga | Canada |
| Rabbit anti-HIV-1 P24 Polyclonal Antibody | Anogen | Mississauga | Canada |
| HIV-1 p24 antibody | ProSci Incorporated | Poway | USA |
| GFP Tag Antibody | Abgent | San Diego | USA |
| pAB Rabbit anti-mouse IgG | Anogen | Mississauga | Canada |
| pAB Rabbit anti-mouse IgG HRP conjugated | Anogen | Mississauga | Canada |
| Monoclonal neutralizing anti-HIV-1 gp41 | Dendritics | Lyon | France |
| Monoclonal neutralizing anti-HIV-1 gp120/160 | Dendritics | Lyon | France |
| Anti-Clostridium tetani toxoid (Tetanus toxoid) | BioPorto | Hellerup | Denmark |
| anti-GFP antibody, monoclonal, from goat | Rockland | Limerick | USA |
| anti-Goat HRP-labelled antibody, polyclonal, f. rabbit | Rockland | Limerick | USA |
