Team:UMaryland/Part Collection
All Parts
All information on our parts can be found on this page
We've also included links to these side pages where we seperate our basic and composite parts
Full Parts List
| Registry | Name | Function | Part Type |
|---|---|---|---|
| K1783000 | pBAD-RBS-Tagged-Miraculin | Makes miraculin containing a transport tag for proper folding and a his-tag for purification | Composite |
| K1783001 | Hok/Sok | Type I Toxin/antitoxin (TA) system, naturally used to maintain plasmids | Composite |
| K1783002 | Constitutive unstable RFP | RFP under a constitutive promoter, with half life of 1 hour | Basic |
| K1783003 | Hok-Sok+CU-RFP | Constitutive unstable RFP, under Hok-Sok regulation. Used as part of tests of Hok-Sok's ability to retain plasmids | Composite |
| K1783004 | ε-cyclase | Converts lycopene into α-carotene | Basic |
| K1783005 | ε-hydroxylase | Adds hydroxyl group to ε ring | Basic |
Combines BBa_K206000 (pBAD promoter) with BBa_K1033120 (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.
Codes for the Hok-Sok Type I toxin-antitoxin system. This system naturally evolved in bacteria as a method to maintain plasmids. Hok-Sok is noted for its presence on the R1 plasmid, which contains genes that confer antibiotic resistance. Hok-Sok is believed to be used to maintain these plasmids in the absence of antibiotic.
Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour.
This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way.
Epsilon cyclase from the A. thaliana genome is capable of converting lycopene to gamma-carotene. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for E. coli.
Epsilon cyclase from the A. thaliana genome is capable of converting alpha-carotene to alpha-cryptoxanthin. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for E. coli.
