Team:METU Turkey
Welcome to iGEM 2015!
Your team has been approved and you are ready to start the iGEM season!
Before you start:
Please read the following pages:
Styling your wiki
As an autoimmune disorder, celiac disorder depends on sensitivity to wheat ingredients such that gliadin which is a glycoprotein complex rich in proline and glutamine, especially alpha-2-gliadin. Alfa-2-glaidin basically bind to of specific receptor called HLA-DQ2 or HLA-DQ8 (because tTG alter the alfa-2-gliadin’s charge density which results in highly negative charges. This negative charge density create significant binding effect of alfa-2-gliadin to HLA-DQ2 OR HLA-DQ8 ). That receptor is an important key component of immune system, which have loci at chromosome 6. Epitopes of that receptor create cellular signaling pathway which results in arise of T and B-cells. These important immune cells eventually kill the specific antigens presented cells. Thus, in order to interfere this autoimmune disorder, protein’s (gluten) specific subunits, which have key roles at celiac disorder are going to be destroyed before the intake of protein in our project. Fundamentally, adding specific enzymes’ genes to E.coli’s genome. Then, E.coli will be added into dough while it is in fermentation process. So during the process of fermentation, specific enzymes’ genes will be transcribed and break down the gluten. To sum up, with the specific enzyme, which is expressed by E.coli, alters and break down the specific protein subunit which is key component of celiac disorder. In addition, we would like to improve the stiffness of gluten-free bread by letting bacteria to secrete additives. For the efficiency of the gluten destruction process, the homogenization of dough will be also improved by the bacteria secretion of additives. Last but not least, receptor complex within E.coli is going to be designed and enable bacteria to express receptor which is like HLA-DQ; in order to detect whether there is a gluten or not.
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
See tips on how to edit your wiki on the Template Documentation page.
Templates
This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the Template Documentation page.
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2015 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
- 2014 SDU Denmark
- 2014 Aalto-Helsinki
- 2014 LMU-Munich
- 2014 Michigan
- 2014 ITESM-Guadalajara
- 2014 SCU-China
Uploading pictures and files
You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)