Team:BABS UNSW Australia/Notebook

Notebook

A brief summary of our trials, tribulations and fleeting moments of joy.

Late November - Early December 2014

    Following a lecture recruitment hustle by Rob, team members applied and were selected for the iGEM team. We all started reading up on synthetic biology and dreaming potential projects ranging from far-fetched to science fiction to milk.

17-19 February 2015

    The team attended a three day bootcamp run by Rob, Daniel and Chris. We learned the basics of molecular biology techniques for the iGEM competition - cloning, enzyme digests, ligations and transformations. We participated in many sessions (some lead by team members) about other aspects of the competition - wiki design, primer design, actual design design. We also started presenting and discussing project ideas, and brainstorming ways to make each of them better.

2-6 March 2015

    The uni semester starts, and we had our first meeting on the first day. We discussed communication strategies (online labbooks, OneNote vs Evernote) and role allocation. Most importantly, we started seriously talking about various projects ideas and their respective advantages and disadvantages.
    Projects discussed included:
    • recombinant dehalogenases
    • dynamic gene editing
    • type 3 secretion systems for protein delivery
    • biophotovoltaic cells
    • synthetic endosymbiotic relationships for therapeutic use (endosynbio!)

    9-13 March 2015

      Despite our 8am Monday morning meeting, we enthusiastically discussed a narrowed down list of projects, and set down pros and cons for each. After some discussion and a preferential vote, we settled on Endosynbio! Despite lying on the sci-fi end of the spectrum, we found some seemingly achievable goals to achieve with our project and decided it was worth the risk. The three parts as we decided were:
      • invasin/listeriolysin
      • colA/minC control system
      • xenophagy
      We also started the iGEM registration process and that was exciting.

      Inspiration

      You can see what others teams have done to organize their notes: