Team:Freiburg/InterLab Study/Details

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Raw-data of InterLab measurements

All data are provided in arbitrary units and were converted to absolute units using the fluorescein standard curve indicated below.

Top10, 10°C

Raw-data from the measurements of Top10 E. coli cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device.

biol 1  biol 2  biol 3  
 tech 1tech 2tech 3tech 1tech 2tech 3tech 1tech 2tech 3
BBa_R0040 (neg)171616661674176917301640234820822141
BBa_J23117240223132331239521962137250322172362
BBa_J23106283202647124464271352448726439252242327722407
BBa_J23101359483383135038446014409342799458024272643200
BBa_I20270 (pos)866280918376943092928647933590129001

Top10, 37°C

Raw-data from the measurements of Top10 E. coli cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device.

biol 1  biol 2  biol 3  
 tech 1tech 2tech 3tech 1tech 2tech 3tech 1tech 2tech 3
BBa_R0040 (neg)252822632313243624042339244822282303
BBa_J23117324330523045275625962585271025282392
BBa_J23106204762061921054218612155321827213101922920067
BBa_J23101642366216159427594525834857278602005655559515
BBa_I20270 (pos)851881427977795883177874850682128800

ArcticExpress, 10°C

Raw-data from the measurements of Arctic Express E. coli cells after incubation for 8h at 37°C and 8h at 10°C. Data are provided in relative fluorescence units as returned by the plate reader device.

biol 1  biol 2  biol 3  
 tech 1tech 2tech 3tech 1tech 2tech 3tech 1tech 2tech 3
BBa_R0040 (neg)233126322235208918781878214319882099
BBa_J23117222822112374257722322475270830432765
BBa_J23106206242016221522179711917821544187761797718296
BBa_J23101407004175343817278872717027771324073015032359
BBa_I20270 (pos)146731397513659135111266613579131551191913791

ArcticExpress, 37°C

Raw-data from the measurements of Top10 E. coli cells after incubation for 16h at 37°C. Data are provided in relative fluorescence units as returned by the plate reader device.

biol 1  biol 2  biol 3  
 tech 1tech 2tech 3tech 1tech 2tech 3tech 1tech 2tech 3
BBa_R0040 (neg)231620862145228520742145231323622147
BBa_J23117278527282791305830303107303827442739
BBa_J23106275822586726231244542393823621203952023520479
BBa_J23101421904047341872356793251232793345293180935964
BBa_I20270 (pos)140001279212803146571454614124132381228612489

Fluorescein standard curve

The data points for 50 ng fluorescein were excluded from standard curve calculation.

ng abs5037,52512,552,510,50
969057615855023264531085252512498119978
994527696754725273481102257642221129054
OVRFLW76097553562841612539667227372955110
Standard curve of sodium fluorescein diluted in 1x phosphate buffered saline. Data points are means of technical triplicates. Error bars indicated the standard deviation.