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  • Team:Oxford/Test/Notebook
    <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>
    500 KB (35,542 words) - 16:53, 18 September 2015
  • Team:Oxford/Notebook
    <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol.
    530 KB (37,526 words) - 03:47, 19 September 2015
  • Team:Oxford/Test/Notebook7
    <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>
    375 KB (27,047 words) - 18:13, 2 September 2015
  • Team:UChicago/Notebook
    need to use high efficiency promoters and lower efficiency RBS. Justin will color:black'>PCR</span></b></p>
    333 KB (47,812 words) - 07:49, 18 September 2015
  • Team:Aix-Marseille/Notebook
    ...h technics, supervised by the instructors. We learned basics, as running a PCR, and where we can find everything needed to work in the lab. Training sessions for PCR
    56 KB (6,991 words) - 00:06, 19 September 2015
  • Team:Oxford/Test/Notebook1
    <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>
    166 KB (11,675 words) - 10:51, 20 August 2015
  • Team:UCLA/Notebook/Protein Cages/17 July 2015
    Introduction: Yesterday, Nithin performed a PCR amplication of our PCquad optimized for iGEM 2.0 with the primers to add th PCR amplification of construct attempt #2
    7 KB (1,116 words) - 23:29, 17 July 2015
  • Team:Toronto/Experiments
    allows for quick growth and high yields. Therefore the proper preparation of LB will be crucial to too high – we want to prevent spills.</li>
    97 KB (16,302 words) - 04:31, 20 November 2015
  • Team:UCSC/Protocols
    <p><strong>Caution:</strong> Gel box has high voltage! Be sure to turn off power pack and unplug leads before removing li Colony PCR Protocols for Transformed <i>E.coli</i>
    68 KB (11,180 words) - 07:32, 18 September 2015
  • Team:EPF Lausanne/Test Scroll
    <li><a href="#colonypcr">Colony PCR</a></li> ...ropdown-toggle" data-toggle="dropdown" href="#">Polymerase Chain Reaction (PCR)<span class="caret"></span></a>
    52 KB (3,960 words) - 11:11, 17 August 2015
  • Team:Macquarie Australia/Notebook1CBP
    <li>PCR for all the single and composite gene parts from 2014 using the protocol sh <li>Q5 high fidelity polymerase 0.25ul</li>
    36 KB (5,999 words) - 03:43, 19 September 2015
  • Team:Aachen/Notebook/Documentation/Glycogen BioBricks
    * gradient PCR using J04450 in pSB1C3 (#ZWDH#) as the template (diluted 1:10) primers are: * run the agarose gel of yesterdays gradient PCR products (#ES4X#) (expected length: 2070&nbsp;bp)
    21 KB (3,097 words) - 19:20, 18 September 2015
  • Team:EPF Lausanne/Notebook/Protocols
    <li><a href="#pcr">Polymerase Chain Reaction (PCR)</a> <li><a href="#colonypcr">Colony PCR</a></li>
    54 KB (5,322 words) - 23:45, 16 September 2015
  • Team:Westminster/Experiments
    <br><a href="#P12">12.PCR Protocols</a> <br><a href="#P13">13.PCR MasterMix</a>
    30 KB (5,210 words) - 11:44, 18 September 2015
  • Team:NU Kazakhstan/Notebook
    <li>PCR (Thermo Scientific Phusion High Fidelity PCR Master-mix):<br> <b>Protocol for PCR reaction:</b><br>
    18 KB (2,876 words) - 00:23, 19 September 2015
  • Team:Carnegie Mellon/Notebook
    ...ge"><b><u>Goal:</u></b></font> To amplify the Gaussia Luciferase DNA using PCR ...<div class = "indentlist"><ul>Use 5µl in PCR</ul></div>
    72 KB (10,158 words) - 20:59, 20 November 2015
  • Team:Toronto/Notebook
    <li><p>PCR amplified todE insert and todF insert using FroggaBio 2X Taq FroggaMix</p> <li>Ran PCR purification of TodE and TodF according to the PureLink PCR Purification protocol.</li>
    21 KB (3,547 words) - 04:32, 20 November 2015
  • Team:China Tongji/Project
    ...="window.scrollTo(0,document.getElementById('fourth2').offsetTop)">4.2 Taq PCR</li> ...="window.scrollTo(0,document.getElementById('fourth3').offsetTop)">4.3 Pfu PCR</li>
    107 KB (14,954 words) - 19:51, 18 September 2015
  • Team:Czech Republic/Protocols
    * Nucleospin Gel and PCR clean-up # Heat in microwave for 1 min at high power.
    13 KB (2,103 words) - 17:03, 18 September 2015
  • Team:Goettingen/Experiments
    ...k() { }); return false;"><h1>PCR product purification using QIAquick&reg; PCR Purification Kit (QIAGEN)</h1></a> ...;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Add 5 volumes Buffer PB to 1 volume of the PCR reaction and mix.</p>
    117 KB (15,375 words) - 00:00, 19 September 2015
  • Team:Brasil-USP/Notebook/protocols
    <li><a href="#directedmutagenesispcr">Directed mutagenesis PCR for restriction site elimination using a plasmid template </a></li> <li><a href="#pcramplification1">PCR amplification (applied to lcp)</a></li>
    24 KB (2,967 words) - 23:52, 20 November 2015
  • Team:Santa Clara/Protocols
    <li>1μl dye to 5μl DNA is sufficient for PCR products and restriction digests.</li> <h4>PCR Components</h4>
    26 KB (2,428 words) - 03:18, 19 September 2015
  • Team:CHINA CD UESTC/Results
    ...e combined <i>mamW+RFP+laccase</i> and <i>RFP</i>+<i>laccase</i> by fusion PCR (Fig. 1B).<br> ...amW</i> and <i>RFP</i> and <i>laccase</i> were amplified by PCR using high fidelity DNA polymerase. <strong>(B)</strong>
    36 KB (3,710 words) - 02:48, 19 September 2015
  • Team:British Columbia/Notebook/Protocols/PhusionPCR
    ...style="width:500px; margin-top:-25px;"><h3>PCR Protocol using Phusion High-Fidelity DNA Polymerase</h3></div> <p><strong>PCR&nbsp;</strong><br />
    8 KB (1,052 words) - 05:45, 22 August 2015
  • Team:Paris Saclay/Experiments
    <li>Pursue the protocol by the NucleoSpin® Gel and PCR Clean-up protocol</li> <h4>3. PCR amplification</h4>
    17 KB (2,170 words) - 23:29, 18 September 2015
  • Team:NU Kazakhstan/InterLab Study
    ...CGCTACTAGTA) Primers. After that, PCR products were purified with QIAquick PCR Purification Kit. <br> <h3>PCR mixture </h3>
    5 KB (804 words) - 04:39, 16 September 2015
  • Team:Peking/Design/Isothermal
    ...nealing and extension, simulating <i>in vivo</i> DNA replication progress. PCR has radically altered molecular biology; via this technique, a trace amount ...ously. All these methods need a thermal cycler; Nested PCR and Multiplexed PCR need more than two pairs of primers, which may lead to the generation of pr
    39 KB (4,417 words) - 14:54, 17 November 2015
  • Team:Oxford/Notebook/Week1
    <p class="text"><a href="#2206c">PCR Set-up</a></p> ...text"><a href="#2206d">Setting up Agarose Gel for Electrophoresis of 22/06 PCR Products</a></p>
    14 KB (1,920 words) - 15:44, 6 July 2015
  • Team:BIT-China/project Fine-regulation circuits.html
    ...of PoxB, Pta, sdhA and some other enzymes could be knocked out to ensure a high succinic acid yield <sup>[15]</sup>. <!-- Error-Prone PCR -->
    43 KB (6,183 words) - 20:20, 18 September 2015
  • Team:UCLA/Notebook
    <li>PCR'd off template</li> <p>PCR Reaction:</p>
    12 KB (1,619 words) - 23:38, 20 May 2015
  • Team:KU Leuven/Notebook/Newsfeed
    ...ks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> ...>, testing colonies by PCR and preparing for sequencing. The high fidelity PCR and cloning of other potential BioBricks was repeated. <br/>
    58 KB (8,236 words) - 10:16, 20 October 2015
  • Team:Freiburg/Protocols/PCR
    <h1 class="sectionedit1"><a name="pcr" id="pcr">PCR</a></h1> <!-- EDIT1 SECTION "PCR" [1-19] -->
    5 KB (690 words) - 07:05, 20 November 2015
  • Team:Hong Kong HKU/Experiments
    <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>
    25 KB (3,384 words) - 14:18, 17 September 2015
  • Team:Example/Experiments/ex
    <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>
    31 KB (4,008 words) - 12:01, 14 September 2015
  • Team:UCLA/Notebook/Interlab Study/14 August 2015
    = PCR Amplification and Gel Extraction of InterLab Devices = == PCR Reaction & Thermocycler Setup ==
    14 KB (1,900 words) - 01:48, 16 August 2015
  • Team:Paris Bettencourt/Protocols
    <div class="textBox" id="PCR"> <h3>PCR protocol</h3>
    37 KB (5,389 words) - 10:32, 19 November 2015
  • Team:UCLA/Notebook/Honeybee Silk/5 May 2015
    ='''Colony PCR of 5/4 Transformation'''= ====Colony PCR Reaction====
    11 KB (1,333 words) - 06:25, 10 July 2015
  • Team:Pasteur Paris/Experiments
    <h4> 1) PCR Amplification using TaKaRa Ex Taq DNA polymerase</h4> <li> Set up the following cycles in a PCR machine
    42 KB (6,658 words) - 21:57, 19 November 2015
  • Team:Freiburg/Project/Methods/Cloning
    <h3 class="sectionedit4">PCR</h3> ...CR approaches were used. In general, PCRs were performed with Phusion High Fidelity DNA Polymerase (NEB). The components of the reaction mixture were used in d
    19 KB (3,058 words) - 16:56, 28 August 2015
  • Team:Aix-Marseille/Protocols
    ...="button" class="btn btn-info" data-toggle="collapse" data-target="#demo9">PCR protocol with EconoTaq PLUS 2X</button> ...fo" data-toggle="collapse" data-target="#demo10">PCR protocol with Q5 High Fidelity DNA Polymerase</button>
    31 KB (3,932 words) - 00:07, 19 September 2015
  • Team:FAFU-CHINA/Notebook
    ...ectively). Then dsDNAs was amplified with four sets of specific primers by PCR. It went through smoothly. So far, we got the target fragments successfully <div style="text-align:left">We picked the colonies to do PCR and sent the positive sequencing(M13).</div>
    18 KB (2,547 words) - 21:19, 18 September 2015
  • Team:NTU-Singapore/Description
    ...the RBS each with only one different nucleotide are designed to carry out PCR. The kit we used allows the whole plasmid to be replicated and the product ...m <i>Psedomonas aeruginosa</i> work well in the chassis of MR-1, giving a high level of electrical output.
    20 KB (2,725 words) - 03:32, 19 September 2015
  • Team:BIT-China/Experiments
    <h2 style="float:left;margin-left:10px;clear:right;" >Error-prone PCR</h2> ...ng the fidelity of the DNA polymerase. This process is similar to standard PCR cloning of a target sequence except that, for constructing a diverse mutage
    7 KB (948 words) - 20:51, 18 September 2015
  • Team:Freiburg/Materials
    <tr><td>PCR cycler</td><td>Gene Amp PCR System 9700</td><td>Applied Biosystems</td><td>Waltham</td><td>USA</td></tr <tr><td>Phusion High-GC Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</t
    14 KB (2,470 words) - 07:00, 20 November 2015
  • Team:UCLA/Notebook/Honeybee Silk/30 May 2015
    ='''Gel Visualization of 5/26 PCR'''= The PCR products from 5/26 were run on a 1% agarose gel against a 1kB ladder. I use
    10 KB (1,234 words) - 22:32, 16 July 2015
  • Team:KU Leuven/Research/Methods
    ...tes, we obtained the double knock-outs. These knock-outs were confirmed by PCR. For more information, please check our result page. <br/> ...nzymes are needed: a mesophylic nuclease, a thermophylic ligase and a high fidelity polymerase. Therefore, the NEBuilder<sup>&#9415;</sup> HiFi DNA Assembly Ma
    45 KB (6,730 words) - 09:34, 20 October 2015
  • Team:UIUC Illinois/Experiments
    ...a lower percentage, such as 0.7% gel, is best for gel extractions, and as high as 2% agarose can be used when resolving large bands of DNA.</li> <h2>I. PCR for Amplification.</h2>
    10 KB (1,713 words) - 03:10, 19 September 2015
  • Team:UCLA/Notebook/Honeybee Silk/26 April 2015
    <summary>4/26 PCR off honey bee gene block</summary> <p>PCR Reaction 1 (q5 polymerase kit): primers honeybee p#7 and honeybee p#8 with
    4 KB (359 words) - 16:33, 5 June 2015
  • Team:UCLA/Notebook/Honeybee Silk/28 April 2015
    <summary> 4/28 PCR to prepare 2 honeybee constructs for cloning</summary> We are repeating the same PCR from 4/26, but only at one temperature instead of a gradient, and we are sc
    3 KB (321 words) - 16:35, 5 June 2015
  • Team:UCLA/Notebook/Honeybee Silk/16 July 2015
    ='''Colony PCR of 7/15 Transformation'''= ===Colony PCR Reaction===
    10 KB (1,301 words) - 01:58, 17 July 2015

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