Difference between revisions of "Team:Freiburg/Protocols/Isoprop Precipitation"
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Revision as of 16:13, 14 September 2015
Isopropanol precipitation
Precipitation and concentration of plasmid DNA from midi-preps
material: 5M NaCl, Isopropanol, 70% EtOH
duration: 60 min
- add 1/10 volume 5M NaCl
- add 1 volume ice cold isopropanol
- incubate for 30 min at -20°C
- centrifuge 30 min, 4°C (pre-cool the centrifuge)
- wash DNA pellet with 70% ethanol
- resuspend in H2O