Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/17 August 2015"

(→‎SDS PAGE: Determining Protein Quality)
 
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='''Redo of Ligation of psb1c3+T7 promoter and Silk+GFP'''=
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{| class="wikitable" style="text-align:center; width:400px; height:200px;"
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|+
 +
|-
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! Component
 +
! Volume (out of 20uL)
 +
|-
 +
! T4 Ligase Reaction Buffer
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| 2 ul
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|-
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! Backbone (psb1c3+t7 promoter)
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| 0.8uL (50 ng)
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|-
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! Insert (silk + spyC)
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| 1.5 ul (146 ng)
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|-
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! H20
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| 14.7 ul
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|-
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! Ligase
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| 1uL
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|}
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*Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C.
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=SDS PAGE: Determining Protein Quality=
 
=SDS PAGE: Determining Protein Quality=
 
*Today we are testing multiple growth conditions of our bl
 
*Today we are testing multiple growth conditions of our bl

Latest revision as of 21:50, 24 August 2015

Redo of Ligation of psb1c3+T7 promoter and Silk+GFP

Component Volume (out of 20uL)
T4 Ligase Reaction Buffer 2 ul
Backbone (psb1c3+t7 promoter) 0.8uL (50 ng)
Insert (silk + spyC) 1.5 ul (146 ng)
H20 14.7 ul
Ligase 1uL
  • Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C.


SDS PAGE: Determining Protein Quality

  • Today we are testing multiple growth conditions of our bl