Team:UCLA/Notebook/Materials
Materials Processing Notebook
Goals
- Wet-Spin fibers from mixtures of silkworm silk and recombinant functionalized spider silk
- Wet-Spin fibers from recombinant honeybee silk
- Construct a post-spin stretch apparatus
- Characterize all spun fibers
- Elastic modulus
- Yield stress
- Beta-sheet content (FTIR)
- Create films from recombinant silk
- Characterize recombinant silk films
- Elastic modulus
- Yield Stress
Achievements
- May 13: completed our practice spins on cellulose acetate!
- May 28: we reconstituted silk solution into fibers!
- July 9: preliminary success with co-spinning b.mori silk w/ recombinant Tamura silk-GFP hybrid
- July 14: a long contiguous co-spin:
- August 19: co-spin with the automated stretcher:
What to accomplish next
- Spin fibers from the recombinant spider silk dopes
Protocols
- [Team:UCLA/Notebook/Materials/degumming | Silk Degumming]
- [Team:UCLA/Notebook/Materials/solubilization | Silk Solubilization]
- Dialysis to remove LiBr
- https://2015.igem.org/Team:UCLA/Notebook/Materials/Dialysis_Concentrate Dialysis to concentrate silk solution]
- Wet Spinning
- Using the Instron
- Using the lyophilizer
- Dissolving silk in HFIP
- Quick Reference
Raw notebook entries
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