Team:UCLA/Attributions

iGEM UCLA





SilkyColi: Reprogramming the physical and functional properties of synthetic silks

Project Attributions

All members of the 2014 and 2015 UCLA iGEM teams were involved in project conceptualization, design, and execution. For each group below, all members were involved in design, implementation, and submission/documentation of all validated BioBricks to the Registry. Additionally, thank you to the 2012 Utah State iGEM Team (ArachniColi) and the Randy Lewis Lab for laying the groundwork for the basic protocols we implemented toward processing our silk samples.

Silk Genetics
Vinson C. Lam and Olivia Cheng

Silk Functionalization
Fasih M. Ahsan, Jessica Jin Huang, and Julian Lutze

Honeybee Silk Synthesis
Daniel Cancilla, Tristan Joseph, and Megan Satyadi

Silk Materials Processing
Michael Cheng, Jared LaMar, and Benjamin Carter Allen

Protein Cages
Phillip Nguyen, William Tyler Lee IV, and Nithin Dharmaraj

InterLab Study
Fasih M. Ahsan and Megan Satyadi

Jamboree Poster Development
Fasih M. Ahsan, Vinson C. Lam, and Daniel Cancilla

Jamboree Presentation Development
Total Team Effort

Wiki/Registry Parts Development
Fasih M. Ahsan and Anuved Verma (UCLA iGEM 2014 Templates)

Outreach/Human Practices
Fasih M. Ahsan, Megan Satyadi, and David Yao

Acknowledgements

Advising:
We thank the following for their guidance and support for the UCLA iGEM team throughout the 2015 season:
Dr. Sriram Kosuri, Assistant Professor, Department of Chemistry and Biochemistry at UCLA, for mentoring the UCLA iGEM program.
Dr. David Breslauer, CSO and Co-Founder, Bolt Threads, for advising our silk materials processing and experimental design strategies.
Dr. Todd Yeates, Professor, Department of Chemistry and Biochemistry at UCLA, for providing advice on the protein cages project.
David Yao, Laboratory Manager, Kosuri Lab, for co-founding and advising the UCLA iGEM team.
Members of the Kosuri Lab for general cloning and lam maintenance assistance (Dr. Hwang Beom Kim, Dr. Rocky Cheung, Eric Jones, Nathan Lubock, Michael Corrado, Kim Insigne, Jess Davis, Guillaume Urtecho,Taylor Ward, and Chris Robles)

Lab Space:
We thank the following for providing essential laboratory space and communal equipment/supplies to conduct our experiments.
Dr. Stephanie Seidlits, Assistant Professor, Department of Bioengineering, for providing their lyophilizer and general teaching lab space.
Dr. Dino Di Carlo Di Carlo, Associate Professor, Department of Bioengineering, for providing storage facility, MilliQ water, and general teaching lab space.
Dr. Benjamin Wu, Associate Professor, Department of Bioengineering, for providing use of their Instron.
Jesse Liang, Graduate Student, Seidlits Lab


Supplies and Troubleshooting Assistance:
We thank the follow for providing essential reagents and supplies during our project.
Dr. Kayvan Niazi, Adjunct Assistant Professor, Department of Bioengineering, for providing our thermocycler and gel imager
Dr. Mark Arbing, for providing protein purification/expression advice and SDS-PAGE equipment/gels
Dr. Ben Wu, Professor and Chairman, Department of Bioengineering, UCLA HSSEAS, for providing use of their instron for materials analysis.

Financial Support:
We thank the following sources for funding this year's project:
UCLA Department of Bioengineering, HSSEAS
UCLA Department of Chemistry and Biochemistry
UCLA DOE Institute
Kosuri Lab
UCLA Department of Microbiology, Immunology, and Molecular Genetics
Bolt Threads
Office of the Dean of Undergraduate Education
Tama Hasson, Vice Provost for Undergraduate Research, UCLA College of Letters and Science
UCLA Undergraduate Research Center - Sciences
Integrated DNA Technologies
UCLA Spark

NHM and Art | Sci Collaboration:
Dr. Victoria Vesna, Professor, Department of Design and Media Arts, UCLA
Mick Lorusso, Installations Director, Art|Sci Center at UCLA CNSI

Jason Fahrion, Los Angeles Artist
Shirley Watts, Natural Discourse
KT Hajeian, Collections Manager - Anthropology, Natural History Museum of Los Angeles County

Administrative Support:
Stacey Tran Fong, Management Services Officer, Department of Bioengineering
Apryll Chin, Fund Manager, Department of Bioengineering