Team:UCLA/Parts
<groupparts>iGEM015 UCLA</groupparts>
Contents
PARTS
Honeybee Parts
- BBa_K1763000 This part is the coding region of honeybee silk protein #3. For the sequence click here . Click on the link for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
- BBa_K1763001 This part is a composite part of the honeybee silk protein #3 coding region along with BBa_R0010 Promoter and RBS BBa_B0034 . For the sequence click here . Click HERE for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
- BBa_K1763008. This sequence is a fusion protein between our honeybee silk and the Spycatcher, protein which has specific affinity for SpyCatcher. It also contains regulatory elements like a promoter and rbs for expression.
- BBa_K1763007 is our honeybee silk sequence with a T7 promoter upstream for effective protein expression in bacterial strains containing t7 polymerase.
- BBa_K1763015 is our honeybee silk sequence+sfGFP with a T7 promoter and RBS upstream that can be used in bacterial strains that contain T7 polymerase.
Spider Silk Parts
Basic ICA Parts: Monomers
- BBa_K1763002: Major Ampullate Spidroin 2, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763003: Major Ampullate Spidroin 2, construct BC. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763004: Major Ampullate Spidroin 2, construct CA. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763009: Major Ampullate Spidroin 2 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.
- BBa_K1763010: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763011: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763012: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
- BBa_K1763423: Major Ampullate Spidroin 1 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.
Spider Silk MaSp ICA Constructs
The following constructs were created using Iterative Capped Assembly (ICA) to assemble MaSp monomers.
- BBa_K1763424: MaSp2-3(1C3)
- BBa_K1763425: MaSp2-3(T7)
- BBa_K1763426: MaSp2-6(1C3)
- BBa_K1763427: MaSp2-6(T7)
- BBa_K1763428: MaSp2-9(1C3)
- BBa_K1763429: MaSp2-9(T7)
- BBa_K1763430: MaSp2-12(1C3)
- BBa_K1763431: MaSp2-12(T7)
- BBa_K1763432: MaSp2-15(1C3)
- BBa_K1763433: MaSp2-15(T7)
- BBa_K1763434: MaSp1-9(1C3)
- BBa_K1763435: MaSp1-9(T7)
- BBa_K1763436: MaSp1-12(1C3)
- BBa_K1763437: MaSp1-12(T7)
- BBa_K1763438: MaSp1/2[2:1]-12(1C3)
- BBa_K1763439: MaSp1/2[2:1]-12(T7)
- BBa_K1763440: MaSp1/2[1:1]-12(1C3)
- BBa_K1763441: MaSp1/2[1:1]-12(T7)
- BBa_K1763442: MaSp1/2[1:2]-12(1C3)
- BBa_K1763443: MaSp1/2[1:2]-12(T7)
Protein Cage Parts
- BBa_K1763421 Protein Cage Mutant 10
Silk Functionalization Parts
- BBa_K1763444: Silk Co-spinning Module