Difference between revisions of "Team:Amsterdam/Project/Phy param/Synechocysytis"

Line 41: Line 41:
 
                             <p>We have demonstrated the instability of classical engineering strategies. As shown in figure 1 the strain SAA023 lose the ability to produce lactate after 300 hours of continuous culture. </p>
 
                             <p>We have demonstrated the instability of classical engineering strategies. As shown in figure 1 the strain SAA023 lose the ability to produce lactate after 300 hours of continuous culture. </p>
 
  <figure>
 
  <figure>
   <img src="https://static.igem.org/mediawiki/2015/3/3b/Amsterdam_qp_lactate.png" alt="The Pulpit Rock" width="304" height="228">
+
   <img src="https://static.igem.org/mediawiki/2015/3/3b/Amsterdam_qp_lactate.png" alt="Lactate Qp and growth">
 
   <figcaption>Fig1. - A view of the pulpit rock in Norway.</figcaption>
 
   <figcaption>Fig1. - A view of the pulpit rock in Norway.</figcaption>
 
</figure>  
 
</figure>  

Revision as of 19:16, 17 September 2015

iGEM Amsterdam 2015

Synechocystis physiological parameters

Counting cells, counting molecules

Overview

Background

Estimating physiological parameters is necessary for building meaningful models that can help us to rationally design our consortium. In addition production stability can be assessed by estimating this parameters over a long period of time give

Aim

We aim to use different cultivation strategies to Characterize growth and production rates of carbon producing Synechocystis strains their stability. .

Main results

We have demonstrated the instability of classical engineering strategies. As shown in figure 1 the strain SAA023 lose the ability to produce lactate after 300 hours of continuous culture.

Lactate Qp and growth
Fig1. - A view of the pulpit rock in Norway.