Difference between revisions of "Team:Pasteur Paris/Notebook"

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<img src="https://static.igem.org/mediawiki/2015/f/f1/Notebook_pasteur2015.jpg" style="width: 100%;"/>

<option value="TPA">TPA</option>

       <div id="week1" class="Transformation Purification Purification Mini_Prep">

       <!-- <i class="tag">Tags: Transformation, Purification</i> -->

              <div class="col-lg-12 week" id="week_1" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/45/Photo_week_1_iGEM_Pasteur.JPG)" onmouseover="Help('We transformed each Biobrick (K808007, K808010, K808011, K808012, K808013, K808014, K936000, 316003) into DH5-alpha bacteria and we performed a bacterial amplification followed by a plasmid purification.', 'week_1')" onmouseout="EfaceHelp()">

       <div id="week2" class="Electrophoresis Gel Purification">

       <!-- <i class="tag">Tags:</i> -->

         <div class="row">        

           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2">

            <div class="col-lg-12 week" id="week_2" style=" background-image:url(https://static.igem.org/mediawiki/2015/1/1c/Week2photo.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_2')" onmouseout="EfaceHelp()">

              Week 2 <p>06/08 - 06/12</p>

            </div>

          </a>

       <div id="week3">

       <!-- <i class="tag">Tags: PCR, Wiki</i> -->

         <div class="col-lg-12 week" id="week_3" style=" background-image:url(https://static.igem.org/mediawiki/2015/6/6d/Week3.JPG)" onmouseover="Help('we used this week to realise some bibliographic researches', 'week_3')" onmouseout="EfaceHelp()">  

              Week 3 <p>06/15 - 06/19</p>

            </div>

        </br>

       <div id="week5" class="Mini_Prep Culture Transformation Dosage Gel Stab_Culture Digestion Migration">

       <!-- <i class="tag">Tags: Wiki</i> -->

            <div class="col-lg-12 week" id="week_5" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b6/Week12.JPG)" onmouseover="Help('We transformed each Biobrick that we had received (K1095000) and verified that we had recieved the right biobrick by digesting the plasmid and performing an electrophoresis on agarose gel and we did a plasmid purification. We performed stab cultures (BBa_808007, 808010, 808011, 808012, 808013, 808014, 316003, 936000, 936013).', 'week_5')" onmouseout="EfaceHelp()">

              Week 5 <p>06/29 - 07/03</p>

            </div>

        </div>

        </br>

       <div id="week6" class="Oligo DataBase Stab_Culture InterLab_Study">

       <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_6" style=" background-image:url(https://static.igem.org/mediawiki/2015/e/eb/Week6.JPG)" onmouseover="Help('We prepared oligonucleotides in order to send then for sequencing at MWG Eurofins. We also did another round of stab cultures.', 'week_6')" onmouseout="EfaceHelp()">

              Week 6 <p>07/06 - 07/10</p>

            </div>

        </br>

       <div id="week8" class="Transformation Stab_Culture">

      <!-- <i class="tag">Tags: Sequencing Wiki</i> -->

            <div class="col-lg-12 week" id="week_8" style=" background-image:url(https://static.igem.org/mediawiki/2015/8/88/Week8.JPG)" onmouseover="Help('We performed stab cultures on the biobricks that we already had, in order to store them.', 'week_8')" onmouseout="EfaceHelp()">

              Week 8 <p>07/20 - 07/24</p>

            </div>

        </br>

       <div id="week9" class="Culture">

       <!-- <i class="tag">Tags: Sequencing</i> -->

            <div class="col-lg-12 week" id="week_9" style=" background-image:url(https://static.igem.org/mediawiki/2015/9/9e/DSCN1936.JPG)" onmouseover="Help('We did a bacterial culture of DH5-alpha bacteria harboring the 808030 Biobrick.', 'week_9')" onmouseout="EfaceHelp()">

              Week 9 <p>07/27 - 07/31</p>

            </div>

        </br>

       <div id="week10" class="TPA Minimal_media Digestion Gel_purification Ligation M9_media Agarose_gel">

       <!-- <i class="tag">Tags: PCR</i> -->

            <div class="col-lg-12 week" id="week_10" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/48/Week10.JPG)" onmouseover="Help('The week was spent testing the toxicity, solubility and use of TPA as a carbon source by the bacteria BAP1. In order to do this, we prepared different types of medium. This week, we also purified and ligated BBa_808030 and the plasmid Backbone pDG011 and checking if the ligation had worked. We also prepared the fragments for the Gibson assembly through PCR Amplification or ligation, depending on the fragment.', 'week_10')" onmouseout="EfaceHelp()">

              Week 10 <p>08/03 - 08/07</p>

            </div>

        </br>

       <div id="week11" class="Ligation DO Dissolution Gibson_Assembly Amplification Transformation Gel_migration Digestion Dilution PCR Culture Mini_Prep Gibson_Master_Mix Purification">

       <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_11" style=" background-image:url(https://static.igem.org/mediawiki/2015/2/2c/Week11.JPG)" onmouseover="Help('The Gibson assembly of cluster 2 was performed and we performed a PCR amplification on it. Other fragments needed for the Gibson assembly of the other clusters were prepared. The transformation of the plasmid pDG011 in DH5-alpha cells was performed. We did an electrophoresis on BBa_K808030 and pDG011 in order to perform a gel purification. We also did a plasmid purification of the plasmid pDG011.', 'week_11')" onmouseout="EfaceHelp()">

              Week 11 <p>08/10 - 08/14</p>

            </div>

        </br>

       <div id="week12" class="PCR">

       <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_12" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b2/Week5.JPG)" onmouseover="Help('We prepared the bacterial strains containing pDG011 and BBa_K808030 in prevision of the pNP-Assay. Several conditions of PCR on pSB1C3 before a Gibson assembly. The nicked plasmid pRS415 for Yeast Assembly was prepared. TPA solubility was assayed. ', 'week_12')" onmouseout="EfaceHelp()">

              Week 12 <p>08/17 - 08/21</p>

            </div>

        </br>

    <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_13" style=" background-image:url(https://static.igem.org/mediawiki/2015/c/ca/Week13.jpeg)" onmouseover="Help('This week, we improved the TPA solubility and the Yeast was treated to prepare the Yeast Assembly. ', 'week_13')" onmouseout="EfaceHelp()">

              Week 13 <p>08/24 - 08/28</p>

            </div>

        </div>

        </br>

       <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_14" style=" background-image:url(https://static.igem.org/mediawiki/2015/a/aa/42f1c36c14288e405732346a2810579b.jpg)" onmouseover="Help('The yeast assembly of Cre Recombinase and pRS415 was performed. We assembled our biobrick BBa_1622001. ', 'week_14')" onmouseout="EfaceHelp()">

              Week 14 <p>08/31 - 09/04</p>

            </div>

        </br>

       <!-- <i class="tag">Tags:</i> -->

            <div class="col-lg-12 week" id="week_15" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/02/Week15.JPG)" onmouseover="Help('The Gibson assembly of our BioBrick BBa_1622000 was done.', 'week_15')" onmouseout="EfaceHelp()">

              Week 15 <p>09/07 - 09/11</p>

            </div>

        </div>