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Week 1
06/01 - 06/05
Reception and bacterial storage of Biobricks
- Resuspension of Biobricks (BBa) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
- Storage at -20°C.
- Transformation in DH5-α competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).
- Cells were plated on LB + chloramphenicol 34 µg/ml (Cm 34).
- Incubation overnight at 37°C.
- Storage at 4°C.
- Plasmid purification following the MiniPrep protocol.
BBa |
Concentration (ng/µl) |
Clone 1 |
Clone 2 |
Clone 3 |
808007 |
73.4 |
44.3 |
56.3 |
808010 |
40.8 |
27.5 |
29.5 |
808011 |
28.8 |
30.4 |
18.5 |
808012 |
49.5 |
N/A |
56.5 |
808013 |
18.8 |
26.0 |
15.7 |
808014 |
30.0 |
41.4 |
37.4 |
316003 |
51.5 |
23.5 |
36.1 |
936000 |
15.2 |
17.7 |
13.8 |
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