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Week 4
06/22 - 06/26
Cont. (cf. week 3)
- MiniPrep of BBa_K808026 following the QIAgen MiniPrep protocol.
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
[DNA] C (ng/µl) |
21.5 |
33.8 |
26.7 |
DNA (250 ng) Volume |
11.6 |
7.4 |
9.4 |
Enzymatic Mix Volume |
1.0 |
1.0 |
1.0 |
Buffer 10X Volume |
5.0 |
5.0 |
5.0 |
Water Volume |
32.4 |
36.6 |
34.6 |
Total |
50.0 |
50.0 |
50.0 |
Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.
- OD measurement using a Spectrophotometer:
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
Concentration (ng/µl) |
21.5 |
33.8 |
26.7 |
OD (260 nm) |
0.43 |
0.67 |
0.53 |
OD (260 nm) / OD (280nm) |
1.69 |
1.81 |
1.77 |
OD (260 nm) / OD (230 nm) |
2.64 |
1.56 |
1.86 |
- Gel migration of the BioBrick K808026 on a 0,8% agarose gel (1h at 130V): 
Figure 6: Results of the gel migration (BBa_K808026). Three different colonies (C1-C3 shown).
- Bacterial culture of BBa_K1095000 on a Petri dish LB + Cm 34 µg/ml.
- Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
[DNA] C (ng/µl) |
21.5 |
33.8 |
26.7 |
DNA (250 ng) Volume |
11.6 |
7.4 |
9.4 |
Enzymatic Mix Volume |
1.0 |
1.0 |
1.0 |
Buffer 10X Volume |
5.0 |
5.0 |
5.0 |
Water Volume |
32.4 |
36.6 |
34.6 |
Total |
50.0 |
50.0 |
50.0 |
Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.
- Transformation of plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-α chemically competent cells.
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