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Week 2
06/08 - 06/12
Testing of Biobricks by restriction digest
- Not I enzymatic digestion of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
- Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).
Figure 1: Verification of the plasmid purification.
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316003 |
808007 |
808010 |
808011 |
808012 |
808013 |
808014 |
936000 |
Theoretical size of insert (bp) |
1061 |
4368 |
948 |
1011 |
1242 |
465 |
1506 |
885 |
Inserts present match theoretical values |
no |
yes |
C1/C2 yes
C3 no |
yes |
no |
no |
yes |
no |
Figure 2: Table of the migration gel (cf. figure 1).
- Storage at -20°C of BBa_K808007, BBa_K808014, BBa_K808011 (only for C2/C3).
- Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.
BB |
clone 1 |
clone 2 |
clone 3 |
C (ng/µl) |
260/280 |
260/230 |
C (ng/µl) |
260/280 |
260/230 |
C (ng/µl) |
260/280 |
260/230 |
808007 |
22.60 |
1.99 |
2.04 |
46.90 |
1.92 |
2.09 |
32.30 |
1.90 |
1.79 |
808010 |
13.50 |
1.85 |
1.39 |
57.10 |
1.95 |
2.17 |
31.40 |
1.86 |
1.84 |
808011 |
37.40 |
1.97 |
2.00 |
61.20 |
1.62 |
1.16 |
21.00 |
1.93 |
1.72 |
808012 |
30.20 |
1.93 |
1.94 |
54.90 |
1.91 |
2.12 |
21.10 |
1.94 |
1.81 |
808013 |
11.50 |
1.91 |
1.11 |
9.60 |
1.78 |
1.42 |
13.90 |
1.74 |
1.21 |
808014 |
33.90 |
1.89 |
1.62 |
24.30 |
1.96 |
2.14 |
14.40 |
1.81 |
1.38 |
316003 |
13.60 |
2.04 |
2.38 |
16.50 |
2.10 |
2.41 |
18.80 |
1.94 |
0.74 |
936000 |
18.20 |
1.98 |
1.19 |
13.10 |
1.97 |
0.93 |
11.90 |
2.16 |
1.18 |
Figure 3: Plasmid purification for BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K316003, BBa_K936000.
- Plasmid purification of BBa_K316003, BBa_K808010, BBa_K808012, BBa_K808013, BBa_K936000.
Figure 4: Migration gel of digestion products.
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