Difference between revisions of "Team:Pasteur Paris/Week 13"
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<p></p><br> | <p></p><br> | ||
− | <h3 | + | <center><h3><em>Yeast assembly</em></h3></center></br> |
<ul style="text-align: left"> | <ul style="text-align: left"> | ||
<li>Treatment of the yeast using a homemade Zymolyase solution :</li> | <li>Treatment of the yeast using a homemade Zymolyase solution :</li> | ||
<ol> | <ol> | ||
− | <li> PBS pH 7.5 5 | + | <li> PBS pH 7.5 5 ml</li> |
<li>glucose 5% to make a 2% solution</li> | <li>glucose 5% to make a 2% solution</li> | ||
<li>Zymolyase 0.1 g (0.012-0.022 mg/mL)</li> | <li>Zymolyase 0.1 g (0.012-0.022 mg/mL)</li> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
<p style="text-align: left">MidiPrep has been digested to verified if the DNA correspond to the plasmid of interest or to an other plasmid that is naturally present in yeast. </p> | <p style="text-align: left">MidiPrep has been digested to verified if the DNA correspond to the plasmid of interest or to an other plasmid that is naturally present in yeast. </p> | ||
<ul style="text-align: left"> | <ul style="text-align: left"> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
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<ul style="text-align: left"> | <ul style="text-align: left"> | ||
<li>PCR amplification of pRS415 with a mutagenesis kit using TE 8.1. </li> | <li>PCR amplification of pRS415 with a mutagenesis kit using TE 8.1. </li> | ||
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</ul> | </ul> | ||
<p> </p> | <p> </p> | ||
− | <h3 | + | <center><h3><em>TPA solubility</em></h3></center></br> |
<ul style="text-align: left"> | <ul style="text-align: left"> | ||
<li>improvement of TPA solubility in different conditions : </li> | <li>improvement of TPA solubility in different conditions : </li> | ||
<ol> | <ol> | ||
− | |||
<li>TPA (0.1 g) + Na<sub>2</sub>H<sub>2</sub>PO<sub>4</sub>(0.5 g) in 150 ml of water: complete TPA solubilization. </li> | <li>TPA (0.1 g) + Na<sub>2</sub>H<sub>2</sub>PO<sub>4</sub>(0.5 g) in 150 ml of water: complete TPA solubilization. </li> | ||
<li>TPA (0.1 g) + NaOH(0.5 g) in 150 ml of water: complete TPA solubilization. </li> | <li>TPA (0.1 g) + NaOH(0.5 g) in 150 ml of water: complete TPA solubilization. </li> | ||
− | |||
</ol> | </ol> | ||
<li>Fluorescence measurements : </li> | <li>Fluorescence measurements : </li> |
Latest revision as of 23:47, 3 October 2015
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Week 13 Yeast assembly
MidiPrep has been digested to verified if the DNA correspond to the plasmid of interest or to an other plasmid that is naturally present in yeast.
We can see the presence of a streak about 7500 bp which seems to correspond to our plasmid pRS415.
TPA solubility
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