Difference between revisions of "Team:William and Mary"
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+ | <link href="https://2015.igem.org/Team:Stockholm/fixbootstrap?action=raw&ctype=text/css" rel="stylesheet" type="text/css"/> | ||
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<link href="https://2015.igem.org/Team:William_and_Mary/demo?action=raw&ctype=text/css" rel="stylesheet" type="text/css"/> | <link href="https://2015.igem.org/Team:William_and_Mary/demo?action=raw&ctype=text/css" rel="stylesheet" type="text/css"/> | ||
− | <link href="https://2015.igem.org/ | + | <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:William_and_Mary/jquerybxsliderCSS?action=raw&ctype=text/css" /> |
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<span class="icon-bar bar3"></span> | <span class="icon-bar bar3"></span> | ||
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− | + | <a href="https://2015.igem.org/Team:William_and_Mary" class="navbar-brand">W&M iGEM 2015</a> | |
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</div> | </div> | ||
<div class="collapse navbar-collapse"> | <div class="collapse navbar-collapse"> | ||
<ul class="nav navbar-nav navbar-right navbar-uppercase"> | <ul class="nav navbar-nav navbar-right navbar-uppercase"> | ||
+ | <p> </p> | ||
− | <li | + | <li> |
− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary"> |
− | + | Home | |
</a> | </a> | ||
− | + | </li> | |
− | + | <li> | |
− | + | <a href="https://2015.igem.org/Team:William_and_Mary/Description"> | |
− | <a href=" | + | Project Description |
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</a> | </a> | ||
</li> | </li> | ||
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− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary#parts"> |
− | + | Parts | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Measurement"> |
− | + | Results | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Modeling"> |
− | + | Modeling | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Interlab"> |
− | + | Interlab Study | |
</a> | </a> | ||
</li> | </li> | ||
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− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Collaborations"> |
Collaboration | Collaboration | ||
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− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Software"> |
− | + | Software | |
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</li> | </li> | ||
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− | <a href=" | + | <a href="https://2015.igem.org/Team:William_and_Mary/Practices"> |
− | + | Human Practices | |
</a> | </a> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="" | + | <a href="https://2015.igem.org/Team:William_and_Mary#ref"> |
− | + | References | |
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:William_and_Mary/Medal_Criteria"> | ||
+ | Medal Criteria | ||
+ | </a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:William_and_Mary/Team"> | ||
+ | Team | ||
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</li> | </li> | ||
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− | < | + | <p class="h1WM" style="font-family:WMSlim-Joe">NOISE</p> |
− | <h5>Characterization of promoter- | + | <h5>Characterization of promoter-driven transcriptional noise in E. coli</h5> |
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− | <video class="bgvid" preload="auto" loop="loop" autoplay="false" muted="muted" volume="0"><source src="https://static.igem.org/mediawiki/2015/ | + | <video class="bgvid" preload="auto" loop="loop" autoplay="false" muted="muted" volume="0"><source src="https://static.igem.org/mediawiki/2015/3/39/WMCYNoise.webm" type="video/webm" responsive-src="https://2015.igem.org/Team:William_and_Mary/rubik_responsive.jpg"><source src="https://static.igem.org/mediawiki/2015/a/a7/WMCYNoise.mp4" type="video/mp4">Video not supported</video></div> |
− | <a href="" data-scroll="true" data-id="# | + | <a href="" data-scroll="true" data-id="#projectdesc" class="scroll-arrow hidden-xs hidden-sm"> |
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− | < | + | <p class="h2WM">What is noise?</p> |
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− | <p> | + | <p>As synthetic biologists continue to construct increasingly complex gene regulatory networks, the need for accurate quantitative characterization of their regulatory components becomes more pressing. Despite the BioBrick registry's thorough characterization of the average strength of promoters, there is insufficient description of the variability in their expression. Our project aims to characterize this variability, or noise, for the most commonly used promoters in synthetic biology and provide additional tools for the regulation of these promoters. |
− | <p>Project | + | <p><a href="https://2015.igem.org/Team:William_and_Mary/Description">Read more on our Project Description page.</a> |
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− | <img alt="..." class="parallax-img" src="https:// | + | <img alt="..." class="parallax-img" src="https://static.igem.org/mediawiki/2015/thumb/a/a8/WMlabBench.jpg/800px-WMlabBench.jpg"> |
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− | + | <!-- Section "Our Projects" example 1: 2 Columns Full Width --> | |
− | <div class="section section-we-made-1 section-with-hover" id=" | + | <div class="section section-we-made-1 section-with-hover" id="parts"> |
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− | <div class="title"> | + | <div class="title animate"> |
− | < | + | <p class = "h2WM">Parts</p2> |
− | + | <div class = "description"> <p>In deciding which parts to submit to the iGEM Registry we focused on three main aspects.</p> | |
− | + | <p><i>First:</i> ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project.<br> <i>Second:</i> Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows simple genome integration using 3A or Gibson Assembly. <br> <i>Third:</i> Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM. </p> | |
− | + | </p></div> | |
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− | <div class="col-md- | + | <div class="col-md-3"> |
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− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/3/3e/Allparts.jpeg"> |
− | <a class="over-area color- | + | <a class="over-area color-black" href="https://2015.igem.org/Team:William_and_Mary/Parts"> |
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− | < | + | <h5>All Parts</h5> |
− | <p> | + | <p>A categorized list of all parts.</p> |
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</a> | </a> | ||
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− | <div class="col-md- | + | <div class="col-md-3"> |
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− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/7/7d/WMcollections.jpeg"> |
− | <a class="over-area color-black" href=" | + | <a class="over-area color-black " href="https://2015.igem.org/Team:William_and_Mary/Part_Collection"> |
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− | < | + | <h5>Part Collection</h5> |
− | <p> | + | <p>Guide RNAs for iGEMs top promoters. </p> |
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</a> | </a> | ||
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− | <div class="col-md- | + | <div class="col-md-3"> |
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− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/7/7d/WMbasic.jpeg"> |
− | <a class="over-area color- | + | <a class="over-area color-black " href="https://2015.igem.org/Team:William_and_Mary/Basic_Part"> |
<div class="content"> | <div class="content"> | ||
− | < | + | <h5>Basic Part</h5> |
− | <p> | + | <p>Targeted repression with dCAS9.</p> |
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</a> | </a> | ||
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− | <div class="col-md- | + | <div class="col-md-3"> |
− | <div class="project"> | + | <div class="project animate"> |
− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/f/f4/WMcomposite.jpeg"> |
− | <a class="over-area color- | + | <a class="over-area color-black " href="https://2015.igem.org/Team:William_and_Mary/Composite_Part"> |
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− | < | + | <h5>Composite Part</h5> |
− | <p> | + | <p>An original integrator suite for the E. coli chromosome.</p> |
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− | <!-- End Section "Our Projects" example | + | <!-- End Section "Our Projects" example 1: 2 Columns Full Width --> |
− | <!-- Section "What We do" example 4: Big Title & | + | <!-- Section "What We do" example 4: Big Title & 3 columns icons --> |
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− | < | + | <p class="h2WM">Measurement & Modeling</p> |
− | + | <p>We measured noise in fluorescence data for dual-integrated sets of CFP and YFP under three promoters: BBa_R0010, BBa_R0011, and BBa_R0051. We also developed an analytic model of the impact of plasmid copy number fluctuations on transcriptional noise, which revealed that intrinsic noise cannot be accurately measured from reporters on the pSB1X3 plasmid series.</p> | |
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− | < | + | <a href = "https://2015.igem.org/Team:William_and_Mary/Measurement"><img alt="Click to go to Measurement" src="https://static.igem.org/mediawiki/2015/0/0f/WMRulerIconColor.png" height= "100" width= "150"/></a> |
</div> | </div> | ||
− | <h3> | + | <h3><a href = "https://2015.igem.org/Team:William_and_Mary/Measurement">Data & Analysis</a></h3> |
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− | <div class="col-md- | + | <div class="col-md-6"> |
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− | < | + | <a href = "https://2015.igem.org/Team:William_and_Mary/Modeling"><img alt="Click to go to the Model." src="https://static.igem.org/mediawiki/2015/7/72/WMNormalIconColor.png" height= "100" width= "150"/></a> |
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− | <h3> | + | <h3><a href = "https://2015.igem.org/Team:William_and_Mary/Modeling">Plasmid Fluctuation Model</a></h3> |
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<!-- End Section "What We do" example 3: Small Title & 3 columns icons --> | <!-- End Section "What We do" example 3: Small Title & 3 columns icons --> | ||
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− | <!-- Section " | + | <!-- Section "What We do" example 3: Small Title & 3 columns icons --> |
− | <div class="section section- | + | <div class="section section-we-do-2" id="workflow2"> |
<div class="container"> | <div class="container"> | ||
− | <div class=" | + | <div class="row"> |
<div class="title"> | <div class="title"> | ||
− | < | + | <p class = "h2WM">Human practices</p> |
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− | + | <p>Our <a href="https://2015.igem.org/Team:William_and_Mary/Practices">Human Practices effort</a> was a multi-faceted outreach approach to science literacy, focusing specifically on spreading a basic understanding of synthetic biology to the general public. We collaborated with numerous organizations to host nine educational Synthetic Biology workshops for the public (from first graders to adults!) and to implement our <a href="https://2015.igem.org/Team:William_and_Mary/Practices#curriculum">educational 24-activity Synthetic Biology booklet</a> into schools worldwide, to further sustain our efforts for years to come.</p> | |
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− | + | <a href="https://2015.igem.org/Team:William_and_Mary/Practices"><img alt="..." src="https://static.igem.org/mediawiki/2015/2/28/WMSpeechIconColor.png" height= "100" width= "100"/></a> | |
− | + | </div> | |
− | </ | + | <h3><a href="https://2015.igem.org/Team:William_and_Mary/Practices">Adult Communication</a></h3> |
+ | </div> | ||
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− | + | <div class="col-md-4"> | |
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− | + | <a href="https://2015.igem.org/Team:William_and_Mary/Practices"><img alt="..." src="https://static.igem.org/mediawiki/2015/0/07/WMBooksIconColor.png" height= "100" width= "100"/></a> | |
− | + | </div> | |
− | + | <h3><a href="https://2015.igem.org/Team:William_and_Mary/Practices">Youth Education</a></h3> | |
− | </ | + | </div> |
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− | + | <a href="https://2015.igem.org/Team:William_and_Mary/Practices"><img alt="..." src="https://static.igem.org/mediawiki/2015/3/3e/WMTimeIconColor.png" height= "100" width= "100"/></a> | |
− | + | </div> | |
− | + | <h3><a href="https://2015.igem.org/Team:William_and_Mary/Practices">Long-Term Sustainability</a></h3> | |
− | </ | + | </div> |
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− | <!-- End Section " | + | <!-- End Section "What We do" example 3: Small Title & 3 columns icons --> |
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− | < | + | <p class="h2WM">Collaboration</p> |
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− | <p> | + | <p>W&M iGEM met and exceeded iGEM's collaboration requirements by collaborating with other researchers in four main ways: creating a pen pal program to connect teams with similar projects, participating in the interlab measurement study, interviewing the general public to provide data to future teams about how to communicate synthetic biology, and collaborating on individual research projects with iGEM teams from University of Georgia, University of Maryland, and Cambridge. </p> |
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− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/c/c8/WmInter.jpg"> |
− | <a class="over-area color-green" href=" | + | <a class="over-area color-green" href="https://2015.igem.org/Team:William_and_Mary/Interlab"> |
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− | <img alt="..." src=" | + | <img alt="..." src="https://static.igem.org/mediawiki/2015/6/6f/Wmpenpal.jpg"> |
− | <a class="over-area color-black" href=" | + | <a class="over-area color-black" href="https://2015.igem.org/Team:William_and_Mary/Collaborations#penpals"> |
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− | <a class="over-area color-blue " href=" | + | <a class="over-area color-blue " href="https://2015.igem.org/Team:William_and_Mary/Collaborations#otherteams"> |
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− | <h4> | + | <h4>With Other Teams</h4> |
− | <p>Click for details. | + | <p>Click for details.</p> |
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+ | <p><a href="https://2015.igem.org/Team:William_and_Mary/Medal_Criteria">We're very proud of all of our accomplishments from over the summer! Click here to learn more about what we did to reach our goals.</a></p> | ||
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Latest revision as of 03:18, 23 November 2015
NOISE Parts
In deciding which parts to submit to the iGEM Registry we focused on three main aspects. First: ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. Measurement & Modeling We measured noise in fluorescence data for dual-integrated sets of CFP and YFP under three promoters: BBa_R0010, BBa_R0011, and BBa_R0051. We also developed an analytic model of the impact of plasmid copy number fluctuations on transcriptional noise, which revealed that intrinsic noise cannot be accurately measured from reporters on the pSB1X3 plasmid series. Human practices Our Human Practices effort was a multi-faceted outreach approach to science literacy, focusing specifically on spreading a basic understanding of synthetic biology to the general public. We collaborated with numerous organizations to host nine educational Synthetic Biology workshops for the public (from first graders to adults!) and to implement our educational 24-activity Synthetic Biology booklet into schools worldwide, to further sustain our efforts for years to come. Collaboration W&M iGEM met and exceeded iGEM's collaboration requirements by collaborating with other researchers in four main ways: creating a pen pal program to connect teams with similar projects, participating in the interlab measurement study, interviewing the general public to provide data to future teams about how to communicate synthetic biology, and collaborating on individual research projects with iGEM teams from University of Georgia, University of Maryland, and Cambridge. 2015 Jamboree Results Undergraduate Grand Prize Winner ReferencesCharacterization of promoter-driven transcriptional noise in E. coli
Second: Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows simple genome integration using 3A or Gibson Assembly.
Third: Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM.
Best in Track: Measurement
Best Education & Public Engagement
Best Presentation
Nominee: Best Mathematical Model