Difference between revisions of "Team:Cambridge-JIC/Tech Specs"
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<h3>Optics</h3> | <h3>Optics</h3> | ||
− | <p><img src="//2015.igem.org/wiki/images/2/2d/CamJIC-Specs-Resolution.png" style="height:300px; margin: 5px;float:right">The imaging system of OpenScope is based on a Raspberry Pi camera. Inverting the lens of the camera converts it into a microscope objective. For a detailed explanation, see the PDF below. With the Raspberry Pi Camera a resolution of 4 μm was achieved, but subsequently sub-micron resolution was reached with a commercially available 'ball lens' (from < | + | <p><img src="//2015.igem.org/wiki/images/2/2d/CamJIC-Specs-Resolution.png" style="height:300px; margin: 5px;float:right">The imaging system of OpenScope is based on a Raspberry Pi camera. Inverting the lens of the camera converts it into a microscope objective. For a detailed explanation, see the PDF below. With the Raspberry Pi Camera a resolution of 4 μm was achieved, but subsequently sub-micron resolution was reached with a commercially available 'ball lens' (from <a href="http://www.comaroptics.com/" class="blue">Comar Optics</a>). OpenScope's objective holder is 3D-printed - like the rest of the chassis - and the spacing between the CCD and the lens in it was optimised for the lenses we used. However, this is an easily modifiable parameter in our .scad design files, which means that anyone can adapt the objective they use for the magnification, field of view, and resolution they need.</p><center><a class="btn btn-default" href="#" role="button" style="color:#444;border-color:#fff">download pdf</a></center> |
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<h3>Movement Precision</h3> | <h3>Movement Precision</h3> | ||
− | <p>When designing the OpenScope stage, we have taken advantage of the flexibility of the plastic following the strategy developed by Dr Richard Bowman. The flexure mechanism allows the stage to move horizontally very smoothly, and all care was taken to provide just as smooth vertical movement. Using screws to control the mechanics provides an extra degree of fine-control. In addition, the microscope can be motorized for complete remote operation (again, through the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell">WebShell</a>). We used cheap, low-power (operating at 5V) stepper motors, which are still highly accurate: they are geared to 513 steps per revolution, which translates into approx. 0.6μm movement of the sample per step along the x- and y-axes. For the detailed analysis, see the document below.</p> | + | <p>When designing the OpenScope stage, we have taken advantage of the flexibility of the plastic following the strategy developed by Dr Richard Bowman. The flexure mechanism allows the stage to move horizontally very smoothly, and all care was taken to provide just as smooth vertical movement. Using screws to control the mechanics provides an extra degree of fine-control. In addition, the microscope can be motorized for complete remote operation (again, through the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">WebShell</a>). We used cheap, low-power (operating at 5V) stepper motors, which are still highly accurate: they are geared to 513 steps per revolution, which translates into approx. 0.6μm movement of the sample per step along the x- and y-axes. For the detailed analysis, see the document below.</p> |
<center><a class="btn btn-default" href="#" role="button" style="color:#444;border-color:#fff">download pdf</a></center> | <center><a class="btn btn-default" href="#" role="button" style="color:#444;border-color:#fff">download pdf</a></center> | ||
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<h3>Power</h3> | <h3>Power</h3> | ||
− | <p><img src="//2015.igem.org/wiki/images/4/4f/CamJIC-MoPi.jpg" style="height:300px;margin:5px;float:right">Considering all of the electronics required to run OpenScope, it consumes 10 times less power than just the arc lamp of a typical lab-bench fluorescence microscope. OpenScope can run on battery power for up to 12 hours depending on use. This time was estimated on the basis of a power consumption analysis, as described in the PDF below. To power OpenScope using batteries you will need the MoPi power module for Raspberry Pi and two 9V batteries. We recommend replacing the single 9V cell for a series of 6x1.5 AA batteries for best performance. For this purpose, you can 3D print <a href="http://www.thingiverse.com/thing:331394">this</a> battery holder.</p> | + | <p><img src="//2015.igem.org/wiki/images/4/4f/CamJIC-MoPi.jpg" style="height:300px;margin:5px;float:right">Considering all of the electronics required to run OpenScope, it consumes 10 times less power than just the arc lamp of a typical lab-bench fluorescence microscope. OpenScope can run on battery power for up to 12 hours depending on use. This time was estimated on the basis of a power consumption analysis, as described in the PDF below. To power OpenScope using batteries you will need the MoPi power module for Raspberry Pi and two 9V batteries. We recommend replacing the single 9V cell for a series of 6x1.5 AA batteries for best performance. For this purpose, you can 3D print <a href="http://www.thingiverse.com/thing:331394" class="blue">this</a> battery holder.</p> |
<p>The ability of the OpenScope to run on battery power makes it particularly useful for imaging in the field or in incubators, without the need for a standard power supply.</p> | <p>The ability of the OpenScope to run on battery power makes it particularly useful for imaging in the field or in incubators, without the need for a standard power supply.</p> | ||
<center><a class="btn btn-default" href="//2015.igem.org/wiki/images/7/73/CamJIC-Specs-Power.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> | <center><a class="btn btn-default" href="//2015.igem.org/wiki/images/7/73/CamJIC-Specs-Power.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> | ||
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<h3>Affordable Price</h3> | <h3>Affordable Price</h3> | ||
− | <p>The total cost of OpenScope is determined mainly by the price of the electronics used, given that the plastic 3D-printed components round up to £2 (100g of PLA, £20 per kg). You will also definitely need the Raspberry Pi camera(£15-20), the Raspberry Pi itself (£25), an Arduino PCB (£20) and some LEDs. On top of that are some optional components: motors if you want to control OpenScope remotely and filters if you will be imaging fluorescence. To figure out which components you need and which not, for your own OpenScope, consult our <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell">Make Your Own</a> page. You can download the full Bill of Materials below. The total price stays in the range £50-150, which is two or more orders of magnitude lower than the price of the commercial lab microscopes.</p> | + | <p>The total cost of OpenScope is determined mainly by the price of the electronics used, given that the plastic 3D-printed components round up to £2 (100g of PLA, £20 per kg). You will also definitely need the Raspberry Pi camera(£15-20), the Raspberry Pi itself (£25), an Arduino PCB (£20) and some LEDs. On top of that are some optional components: motors if you want to control OpenScope remotely and filters if you will be imaging fluorescence. To figure out which components you need and which not, for your own OpenScope, consult our <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">Make Your Own</a> page. You can download the full Bill of Materials below. The total price stays in the range £50-150, which is two or more orders of magnitude lower than the price of the commercial lab microscopes.</p> |
<center><a class="btn btn-default" href="#" role="button" style="color:#fff;border-color:#fff;background-color:#444">download pdf</a></center> | <center><a class="btn btn-default" href="#" role="button" style="color:#fff;border-color:#fff;background-color:#444">download pdf</a></center> | ||
</div></div></section> | </div></div></section> |
Revision as of 19:42, 16 September 2015