Difference between revisions of "Team:UCLA/Notebook/Materials/12 May 2015"

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***periodically, an air bubble comes out from the tube -- is this an indication that there is air in the tubing? Air is something that might be providing huge back pressure against the motor's force
 
***periodically, an air bubble comes out from the tube -- is this an indication that there is air in the tubing? Air is something that might be providing huge back pressure against the motor's force
 
***a thin stream can be observed rising from the tip of the tube to the surface of the water. I think that this is acetone leaving the dope. The question is, is acetone leaving the dope that's already in the coagulation bath, or leaving the dope that's inside of the tube? If it's the latter, then that may explain why the motor stops being able to push out the dope.
 
***a thin stream can be observed rising from the tip of the tube to the surface of the water. I think that this is acetone leaving the dope. The question is, is acetone leaving the dope that's already in the coagulation bath, or leaving the dope that's inside of the tube? If it's the latter, then that may explain why the motor stops being able to push out the dope.
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*started a new dialysis, using the Snakeskin dialysis tubing and dialysis clips.  
 
*started a new dialysis, using the Snakeskin dialysis tubing and dialysis clips.  
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**we cut 15 mm of tubing for 10 mL of silk solution. This was waaaaay too big. The dialysis clips allow us to be a bit more conservative on how much tubing we use per mL solution.
 
**start time: 5:15 pm
 
**start time: 5:15 pm
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**1st change: 6:25 pm
  
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*centrifuged the previously dialyzed silk
 
*centrifuged the previously dialyzed silk
 
**note to self: just use a more powerful centrifuge. It isn't worth it to split up the sample into microtubes to spin in our table top centrifuge.
 
**note to self: just use a more powerful centrifuge. It isn't worth it to split up the sample into microtubes to spin in our table top centrifuge.

Revision as of 01:26, 13 May 2015

  • Tried extrusion, this time with PEEK tubing (0.005 inch inner diameter) cut with the tubing cutter
    • tube length was 10 mm
    • extrusion rate of 6 uL/min
    • fibers still don't form. Rather, the dope blobs up upon coming into contact with the water
    • some observations:
      • the syringe pump inevitably reaches a point where it stops pushing. The motor is still working, but the drive screw is clearly not moving at all (and hence, the drive block isn't moving). Curiously, dope still comes out of the tubing when the motor is stalled
      • periodically, an air bubble comes out from the tube -- is this an indication that there is air in the tubing? Air is something that might be providing huge back pressure against the motor's force
      • a thin stream can be observed rising from the tip of the tube to the surface of the water. I think that this is acetone leaving the dope. The question is, is acetone leaving the dope that's already in the coagulation bath, or leaving the dope that's inside of the tube? If it's the latter, then that may explain why the motor stops being able to push out the dope.



  • started a new dialysis, using the Snakeskin dialysis tubing and dialysis clips.
    • we cut 15 mm of tubing for 10 mL of silk solution. This was waaaaay too big. The dialysis clips allow us to be a bit more conservative on how much tubing we use per mL solution.
    • start time: 5:15 pm
    • 1st change: 6:25 pm



  • centrifuged the previously dialyzed silk
    • note to self: just use a more powerful centrifuge. It isn't worth it to split up the sample into microtubes to spin in our table top centrifuge.
    • ended up with 25 mL of silk
    • pipetted out 500 uL to dry to see our silk concentration
      • I am expecting a very low concentration, due to the way that our dialysis was leaking.