Difference between revisions of "Team:Pasteur Paris/Week 1"
Line 122: | Line 122: | ||
<p style="text-align: justify; padding: 30px;"> | <p style="text-align: justify; padding: 30px;"> | ||
+ | |||
<p>1/ Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.<br /> | <p>1/ Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.<br /> |
Revision as of 21:54, 12 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 1
1/ Resuspension of DNA in 15µL of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003. 2/ Storage at -20°C. 3/ Transformation in DH5α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003). 4/ Cells were plated on LB + chloramphenicol 34µg/mL (Cm34). 5/ Incubation overnight. 6/ Storage at 4°C. 7/ Plasmid purification following the MiniPrep protocol.
^ |