Difference between revisions of "Team:Pasteur Paris/Week 4"

Line 120: Line 120:
 
                
 
                
 
<ol>
 
<ol>
−
   <li>MiniPrep of  BBa_808026 following the QIAgen MiniPrep protocol. </li>
+
   <li style="text-align:left">MiniPrep of  BBa_808026 following the QIAgen MiniPrep protocol. </li>
−
</ol>
+
 
<div align="center">
 
<div align="center">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
Line 208: Line 207:
 
   <br />
 
   <br />
 
   <strong><u>Figure 7</u></strong><u>:</u> Results of the  Gel Migration (BBa_808026). </p>
 
   <strong><u>Figure 7</u></strong><u>:</u> Results of the  Gel Migration (BBa_808026). </p>
−
<ol>
+
   <li style="text-align:left">Bacterial culture  of BBa_K1095000 on a Petri dish LB+Cm34. </li>
−
   <li>Bacterial culture  of BBa_K1095000 on a Petri dish LB+Cm34. </li>
+
   <li style="text-align:left">Plasmid  purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li>
−
   <li>Plasmid  purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li>
+
−
</ol>
+
 
<div align="center">
 
<div align="center">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
Line 259: Line 256:
 
</div>
 
</div>
 
<p align="center"><u>Figure 6:</u> DNA Extraction  by using the QiAprep spin protocol for a bioassay and digest. </p>
 
<p align="center"><u>Figure 6:</u> DNA Extraction  by using the QiAprep spin protocol for a bioassay and digest. </p>
−
<ol>
+
   <li style="text-align:left">Transformation of  plasmids TeV and pDG022 in DH5-⍺ chemically competent cells. </li>
−
   <li>Transformation of  plasmids TeV and pDG022 in DH5-⍺ chemically competent cells. </li>
+
 
</ol>
 
</ol>
 
             </td>
 
             </td>

Revision as of 00:12, 13 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 4

06/22 - 06/26

  1. MiniPrep of BBa_808026 following the QIAgen MiniPrep protocol.

    2. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µL)

    21,5

    33,8

    26,7

    DNA (250ng) Volume

    11,6

    7,4

    9,4

    Mix enzymatique Volume

    1

    1

    1

    Tampon 10X Volume

    5

    5

    5

    Water Volume

    32,4

    36,6

    34,6

    Total

    50

    50

    50

    Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.
    - OD measurement using a Spectrophotometer


    BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    Concentration (ng/µL)

    21,5

    33,8

    26,7

    OD(260 nm)

    0,430

    0,665

    0,534

    OD(260 nm)/OD (280nm)

    1,69

    1,81

    1,77

    OD(260 nm)/OD (230nm)

    2,64

    1,56

    1,86

    - Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel. (1h at 130V)l

    Figure 7: Results of the Gel Migration (BBa_808026).

  2. Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.
  3. Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.

    4. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µL)

    21,5

    33,8

    26,7

    DNA (250ng) Volume

    11,6

    7,4

    9,4

    Mix enzymatique Volume

    1

    1

    1

    Tampon 10X Volume

    5

    5

    5

    Water Volume

    32,4

    36,6

    34,6

    Total

    50

    50

    50

    Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.

  4. Transformation of plasmids TeV and pDG022 in DH5-⍺ chemically competent cells.