Difference between revisions of "Team:Pasteur Paris/Week 4"

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<p align="left">- Gel migration of the BioBrick K808026  on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" />
 
<p align="left">- Gel migration of the BioBrick K808026  on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" />
 
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Revision as of 02:50, 13 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 4

06/22 - 06/26




  1. MiniPrep of BBa_808026 following the QIAgen MiniPrep protocol.

    2. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µL)

    21,5

    33,8

    26,7

    DNA (250ng) Volume

    11,6

    7,4

    9,4

    Mix enzymatique Volume

    1

    1

    1

    Tampon 10X Volume

    5

    5

    5

    Water Volume

    32,4

    36,6

    34,6

    Total

    50

    50

    50

    Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.


    - OD measurement using a Spectrophotometer:


    BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    Concentration (ng/µL)

    21,5

    33,8

    26,7

    OD(260 nm)

    0,430

    0,665

    0,534

    OD(260 nm)/OD (280nm)

    1,69

    1,81

    1,77

    OD(260 nm)/OD (230nm)

    2,64

    1,56

    1,86

    - Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):

    l

    Figure 7: Results of the Gel Migration (BBa_808026).

  2. Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.
  3. Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.

    4. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µL)

    21,5

    33,8

    26,7

    DNA (250ng) Volume

    11,6

    7,4

    9,4

    Mix enzymatique Volume

    1

    1

    1

    Tampon 10X Volume

    5

    5

    5

    Water Volume

    32,4

    36,6

    34,6

    Total

    50

    50

    50

    Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.

  4. Transformation of plasmids TeV and pDG022 in DH5-⍺ chemically competent cells.