Difference between revisions of "Team:Pasteur Paris/Week 1"

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   <table border="1" cellspacing="0" cellpadding="0" width="620">
 
   <table border="1" cellspacing="0" cellpadding="0" width="620">
 
     <tr>
 
     <tr>
       <td width="100" rowspan="2" valign="top"><p align="center"><strong>BBa</strong></p></td>
+
       <td width="100" rowspan="2" valign="center"><p align="center"><strong>BBa</strong></p></td>
       <td width="520" colspan="3" valign="top"><p align="center"><strong>Concentration   (ng/µL)</strong></p></td>
+
       <td width="520" colspan="3" valign="top"><p align="center"><strong>Concentration (ng/µl)</strong></p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
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       <td width="100" valign="top"><p align="center"><strong>808013</strong></p></td>
 
       <td width="100" valign="top"><p align="center"><strong>808013</strong></p></td>
 
       <td width="173" valign="top"><p align="center">18.8</p></td>
 
       <td width="173" valign="top"><p align="center">18.8</p></td>
       <td width="173" valign="top"><p align="center">26</p></td>
+
       <td width="173" valign="top"><p align="center">26.0</p></td>
 
       <td width="173" valign="top"><p align="center">15.7</p></td>
 
       <td width="173" valign="top"><p align="center">15.7</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td width="100" valign="top"><p align="center"><strong>808014</strong></p></td>
 
       <td width="100" valign="top"><p align="center"><strong>808014</strong></p></td>
       <td width="173" valign="top"><p align="center">30</p></td>
+
       <td width="173" valign="top"><p align="center">30.0</p></td>
 
       <td width="173" valign="top"><p align="center">41.4</p></td>
 
       <td width="173" valign="top"><p align="center">41.4</p></td>
 
       <td width="173" valign="top"><p align="center">37.4</p></td>
 
       <td width="173" valign="top"><p align="center">37.4</p></td>

Revision as of 19:31, 17 September 2015

Week 1

06/01 - 06/05




  1. Resuspension of Biobrick (BBa) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.

  2. Storage at -20°C.

  3. Transformation in DH5α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

  4. Cells were plated on LB + chloramphenicol 34 µg/ml (Cm34).

  5. Incubation overnight at 37°C.

  6. Storage at 4°C.

  7. Plasmid purification following the MiniPrep protocol.

BBa

Concentration (ng/µl)

Clone 1

Clone 2

Clone 3

808007

73.4

44.3

56.3

808010

40.8

27.5

29.5

808011

28.8

30.4

18.5

808012

49.5

N/A

56.5

808013

18.8

26.0

15.7

808014

30.0

41.4

37.4

316003

51.5

23.5

36.1

936000

15.2

17.7

13.8

 



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