Difference between revisions of "Team:Pasteur Paris/Week 4"

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   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">BBa_K808026 </p></td>
+
       <td width="197" valign="center"><p align="center">BBa_K808026 </p></td>
 
       <td width="60" valign="top"><p align="center">Clone 1 </p></td>
 
       <td width="60" valign="top"><p align="center">Clone 1 </p></td>
 
       <td width="64" valign="top"><p align="center">Clone 2 </p></td>
 
       <td width="64" valign="top"><p align="center">Clone 2 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">DNA (250ng) Volume </p></td>
+
       <td width="197" valign="top"><p align="center">DNA (250 ng) Volume </p></td>
 
       <td width="60" valign="top"><p align="center">11.6 </p></td>
 
       <td width="60" valign="top"><p align="center">11.6 </p></td>
 
       <td width="64" valign="top"><p align="center">7.4 </p></td>
 
       <td width="64" valign="top"><p align="center">7.4 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">Tampon 10X Volume </p></td>
+
       <td width="197" valign="top"><p align="center">Buffer 10X Volume </p></td>
 
       <td width="60" valign="top"><p align="center">5.0 </p></td>
 
       <td width="60" valign="top"><p align="center">5.0 </p></td>
 
       <td width="64" valign="top"><p align="center">5.0 </p></td>
 
       <td width="64" valign="top"><p align="center">5.0 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="219" valign="top"><p align="center">Concentration (ng/µL)</p></td>
+
       <td width="219" valign="top"><p align="center">Concentration (ng/µl)</p></td>
 
       <td width="76" valign="top"><p align="center">21.5</p></td>
 
       <td width="76" valign="top"><p align="center">21.5</p></td>
 
       <td width="79" valign="top"><p align="center">33.8</p></td>
 
       <td width="79" valign="top"><p align="center">33.8</p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="219" valign="top"><p align="center">OD(260 nm)</p></td>
+
       <td width="219" valign="top"><p align="center">OD (260 nm)</p></td>
−
       <td width="76" valign="top"><p align="center">0.430</p></td>
+
       <td width="76" valign="top"><p align="center">0.43</p></td>
−
       <td width="79" valign="top"><p align="center">0.665</p></td>
+
       <td width="79" valign="top"><p align="center">0.67</p></td>
−
       <td width="80" valign="top"><p align="center">0.534</p></td>
+
       <td width="80" valign="top"><p align="center">0.53</p></td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="219" valign="top"><p align="center">OD(260 nm)/OD (280nm)</p></td>
+
       <td width="219" valign="top"><p align="center">OD (260 nm) / OD (280nm)</p></td>
 
       <td width="76" valign="top"><p align="center">1.69</p></td>
 
       <td width="76" valign="top"><p align="center">1.69</p></td>
 
       <td width="79" valign="top"><p align="center">1.81</p></td>
 
       <td width="79" valign="top"><p align="center">1.81</p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="219" valign="top"><p align="center">OD(260 nm)/OD (230nm)</p></td>
+
       <td width="219" valign="top"><p align="center">OD (260 nm) / OD (230 nm)</p></td>
 
       <td width="76" valign="top"><p align="center">2.64</p></td>
 
       <td width="76" valign="top"><p align="center">2.64</p></td>
 
       <td width="79" valign="top"><p align="center">1.56</p></td>
 
       <td width="79" valign="top"><p align="center">1.56</p></td>
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<br>
 
<br>
 
<p align="left">- Gel migration of the BioBrick K808026  on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" />
 
<p align="left">- Gel migration of the BioBrick K808026  on a 0,8% Agarose Gel (1h at 130V):</p> <img src="https://static.igem.org/mediawiki/2015/4/4a/IGEM_Pasteur_gel_3_week_4.png" alt="l" width="449" height="336" /><br clear="all" />
−
   <strong><u>Figure 6</u></strong><u>:</u> Results of the  Gel Migration (BBa_808026). </p><br>
+
   <strong><u>Figure 6</u></strong><u>:</u> Results of the  gel migration (BBa_808026). </p><br>
 
   <li style="text-align:left">Bacterial culture  of BBa_K1095000 on a Petri dish LB+Cm34. </li><br>
 
   <li style="text-align:left">Bacterial culture  of BBa_K1095000 on a Petri dish LB+Cm34. </li><br>
 
   <li style="text-align:left">Plasmid  purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li>
 
   <li style="text-align:left">Plasmid  purification of BBa_808026 following the MidiPrep and MiniPrep protocol. </li>
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   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
   <table border="1" cellspacing="0" cellpadding="0" width="382">
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">BBa_K808026 </p></td>
+
       <td width="197" valign="center"><p align="center">BBa_K808026 </p></td>
 
       <td width="60" valign="top"><p align="center">Clone 1 </p></td>
 
       <td width="60" valign="top"><p align="center">Clone 1 </p></td>
 
       <td width="64" valign="top"><p align="center">Clone 2 </p></td>
 
       <td width="64" valign="top"><p align="center">Clone 2 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">[DNA] C (ng/µL) </p></td>
+
       <td width="197" valign="top"><p align="center">[DNA] C (ng/µl) </p></td>
 
       <td width="60" valign="top"><p align="center">21.5 </p></td>
 
       <td width="60" valign="top"><p align="center">21.5 </p></td>
 
       <td width="64" valign="top"><p align="center">33.8 </p></td>
 
       <td width="64" valign="top"><p align="center">33.8 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">DNA (250ng) Volume </p></td>
+
       <td width="197" valign="top"><p align="center">DNA (250 ng) Volume </p></td>
 
       <td width="60" valign="top"><p align="center">11.6 </p></td>
 
       <td width="60" valign="top"><p align="center">11.6 </p></td>
 
       <td width="64" valign="top"><p align="center">7.4 </p></td>
 
       <td width="64" valign="top"><p align="center">7.4 </p></td>
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     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td width="197" valign="top"><p align="center">Tampon 10X Volume </p></td>
+
       <td width="197" valign="top"><p align="center">Buffer 10X Volume </p></td>
 
       <td width="60" valign="top"><p align="center">5.0 </p></td>
 
       <td width="60" valign="top"><p align="center">5.0 </p></td>
 
       <td width="64" valign="top"><p align="center">5.0 </p></td>
 
       <td width="64" valign="top"><p align="center">5.0 </p></td>

Revision as of 19:46, 17 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 4

06/22 - 06/26




  1. MiniPrep of BBa_808026 following the QIAgen MiniPrep protocol.

    2. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µl)

    21.5

    33.8

    26.7

    DNA (250 ng) Volume

    11.6

    7.4

    9.4

    Enzymatic Mix Volume

    1.0

    1.0

    1.0

    Buffer 10X Volume

    5.0

    5.0

    5.0

    Water Volume

    32.4

    36.6

    34.6

    Total

    50.0

    50.0

    50.0

    Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.


    - OD measurement using a Spectrophotometer:


    BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    Concentration (ng/µl)

    21.5

    33.8

    26.7

    OD (260 nm)

    0.43

    0.67

    0.53

    OD (260 nm) / OD (280nm)

    1.69

    1.81

    1.77

    OD (260 nm) / OD (230 nm)

    2.64

    1.56

    1.86

    - Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):

    l
    Figure 6: Results of the gel migration (BBa_808026).


  2. Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.

  3. Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.

    4. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µl)

    21.5

    33.8

    26.7

    DNA (250 ng) Volume

    11.6

    7.4

    9.4

    Enzymatic Mix Volume

    1.0

    1.0

    1.0

    Buffer 10X Volume

    5.0

    5.0

    5.0

    Water Volume

    32.4

    36.6

    34.6

    Total

    50.0

    50.0

    50.0

    Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.


  4. Transformation of plasmids TeV and pDG022 in DH5-⍺ chemically competent cells.