Difference between revisions of "Team:Pasteur Paris/Week 4"

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     <tr>
 
     <tr>
 
       <td width="219" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td>
 
       <td width="219" valign="center"><p align="center"><strong>BBa_K808026</strong> </p></td>
−
       <td width="76" valign="top"><p align="center"><strong>Clone 1 </strong></p></td>
+
       <td width="90" valign="top"><p align="center"><strong>Clone 1 </strong></p></td>
−
       <td width="79" valign="top"><p align="center"><strong>Clone 2 </strong></p></td>
+
       <td width="80" valign="top"><p align="center"><strong>Clone 2 </strong></p></td>
 
       <td width="80" valign="top"><p align="center"><strong>Clone 3 </strong></p></td>
 
       <td width="80" valign="top"><p align="center"><strong>Clone 3 </strong></p></td>
 
     </tr>
 
     </tr>
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</div>
 
</div>
 
<p align="center"><strong><u>Figure 7:</u></strong> DNA Extraction  by using the QiAprep spin protocol for a bioassay and digest. </p><br>
 
<p align="center"><strong><u>Figure 7:</u></strong> DNA Extraction  by using the QiAprep spin protocol for a bioassay and digest. </p><br>
−
   <li style="text-align:left">Transformation of  plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-⍺ chemically competent cells. </li>
+
   <li style="text-align:left">Transformation of  plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-α chemically competent cells. </li>
 
</ol>
 
</ol>
 
             </td>
 
             </td>

Revision as of 23:31, 17 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 4

06/22 - 06/26




  1. MiniPrep of BBa_K808026 following the QIAgen MiniPrep protocol.

    2. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µl)

    21.5

    33.8

    26.7

    DNA (250 ng) Volume

    11.6

    7.4

    9.4

    Enzymatic Mix Volume

    1.0

    1.0

    1.0

    Buffer 10X Volume

    5.0

    5.0

    5.0

    Water Volume

    32.4

    36.6

    34.6

    Total

    50.0

    50.0

    50.0

    Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.


    - OD measurement using a Spectrophotometer:


    BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    Concentration (ng/µl)

    21.5

    33.8

    26.7

    OD (260 nm)

    0.43

    0.67

    0.53

    OD (260 nm) / OD (280nm)

    1.69

    1.81

    1.77

    OD (260 nm) / OD (230 nm)

    2.64

    1.56

    1.86

    - Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):

    l
    Figure 6: Results of the gel migration (BBa_808026). Three different colonies (C1-C3 shown)


  2. Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.

  3. Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.

    4. BBa_K808026

    Clone 1

    Clone 2

    Clone 3

    [DNA] C (ng/µl)

    21.5

    33.8

    26.7

    DNA (250 ng) Volume

    11.6

    7.4

    9.4

    Enzymatic Mix Volume

    1.0

    1.0

    1.0

    Buffer 10X Volume

    5.0

    5.0

    5.0

    Water Volume

    32.4

    36.6

    34.6

    Total

    50.0

    50.0

    50.0

    Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.


  4. Transformation of plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-α chemically competent cells.