Difference between revisions of "Team:Pasteur Paris/Week 10"

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<!--<h3><em>Toxicity and solubility of TPA (Terephthalic acid)</em></h3><br>
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   <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li>
 
   <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li>

Revision as of 12:12, 18 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 10

08/03 - 08/07




Enzymatic Assay of NB-Esterase


  1. XbaI ans PstI enzymatic digest of the Biobrick BBa_K808030 and pDG011 plasmid.
  2. Gel purification of the digested fragments on 0.8% agarose gel.
  3. Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase.
  4. Gel migration on 0.8% agarose gel.
    5. k
    Figure 8: Gel for verification of ligation.
    There is no band for the ligation sample.


    Gibson Assembly


  5. Ligation of ThpA1, Transp_, 1392932_, using T4 DNA ligase.
  6. DNA concentration measurement using a spectrophotometer.

Biobrick

DNA Concentration (ng/µl)

OD (230 nm)

OD (260 nm)

OD (280 nm)

OD (260 nm) / OD (280 nm)

OD (260 nm) / OD (230 nm)

808010

30.00

9.33

0.75

0,30

1.87

0.06

808011

50.00

11.78

1.00

0.63

1.61

0.09

808012

17.50

10.93

0.33

0.18

0.84

0.03

808013

5.00

10.55

0.10

0.06

1.56

0.01

80801v

22.5

12.13

0.47

0.30

1.54

0.04

1095000

37.50

18.48

0.78

0.43

1.83

0.04