Week 4

06/22 - 06/26

1. MiniPrep of BBa_K808026 following the QIAgen MiniPrep protocol.



BBa_K808026	Clone 1	Clone 2	Clone 3
[DNA] C (ng/µl)	21.5	33.8	26.7
DNA (250 ng) Volume	11.6	7.4	9.4
Enzymatic Mix Volume	1.0	1.0	1.0
Buffer 10X Volume	5.0	5.0	5.0
Water Volume	32.4	36.6	34.6
Total	50.0	50.0	50.0

Figure 5: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.




- OD measurement using a Spectrophotometer:

BBa_K808026	Clone 1	Clone 2	Clone 3
Concentration (ng/µl)	21.5	33.8	26.7
OD (260 nm)	0.43	0.67	0.53
OD (260 nm) / OD (280nm)	1.69	1.81	1.77
OD (260 nm) / OD (230 nm)	2.64	1.56	1.86




- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel (1h at 130V):


Figure 6: Results of the gel migration (BBa_808026). Three different colonies (C1-C3 shown).


2. Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.


3. Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.



BBa_K808026	Clone 1	Clone 2	Clone 3
[DNA] C (ng/µl)	21.5	33.8	26.7
DNA (250 ng) Volume	11.6	7.4	9.4
Enzymatic Mix Volume	1.0	1.0	1.0
Buffer 10X Volume	5.0	5.0	5.0
Water Volume	32.4	36.6	34.6
Total	50.0	50.0	50.0

Figure 7: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.



4. Transformation of plasmids TeV protease and pDG022 plasmid (for protein expression) in DH5-α chemically competent cells.