Difference between revisions of "Team:Pasteur Paris/Week 1"
| Line 110: | Line 110: | ||
<li style="text-align:left">Resuspension of Biobricks (BBa) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.</li><br> | <li style="text-align:left">Resuspension of Biobricks (BBa) DNA in 15 µl of DNAse, RNAse free water: BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.</li><br> | ||
<li style="text-align:left">Storage at -20°C.</li><br> | <li style="text-align:left">Storage at -20°C.</li><br> | ||
| − | <li style="text-align:left">Transformation in | + | <li style="text-align:left">Transformation in DH5-α Competent cells purchased from Invitrogen (# 18265-017) was done following the transformation protocol. (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).</li><br> |
<li style="text-align:left">Cells were plated on LB + chloramphenicol 34 µg/ml (Cm34). </li><br> | <li style="text-align:left">Cells were plated on LB + chloramphenicol 34 µg/ml (Cm34). </li><br> | ||
<li style="text-align:left">Incubation overnight at 37°C. </li><br> | <li style="text-align:left">Incubation overnight at 37°C. </li><br> | ||
Revision as of 12:33, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 1
^ |
|||||||||||||||||||||||||||||||||||||||