Difference between revisions of "Team:Pasteur Paris/Week 10"
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<h3><em>Toxicity and solubility of TPA (Terephthalic acid)</em></h3><br> | <h3><em>Toxicity and solubility of TPA (Terephthalic acid)</em></h3><br> | ||
<ol> | <ol> | ||
− | <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li> | + | <li style="text-align:left">Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l </li></br> |
− | <li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li> | + | <li style="text-align:left">Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l </li></br> |
− | <li style="text-align:left">Solubilization of TPA by NaOH</li> | + | <li style="text-align:left">Solubilization of TPA by NaOH</li></br> |
− | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li> | + | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li></br> |
− | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li> | + | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> |
− | <li style="text-align:left"><u>Preparation of different media containing TPA and EG:</u></li> | + | <li style="text-align:left"><u>Preparation of different media containing TPA and EG:</u></li></br> |
<li style="text-align:left">Media 1 to 10 are made in solid form:</li> | <li style="text-align:left">Media 1 to 10 are made in solid form:</li> | ||
− | < | + | <ol> |
<li style="text-align:left">M9</li> | <li style="text-align:left">M9</li> | ||
<li style="text-align:left">M9 + TPA (1.2mM)</li> | <li style="text-align:left">M9 + TPA (1.2mM)</li> | ||
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<li style="text-align:left">M9 + sucrose + TPA (6mM)</li> | <li style="text-align:left">M9 + sucrose + TPA (6mM)</li> | ||
<li style="text-align:left">M9 + sucrose + TPA (1,2mM)</li> | <li style="text-align:left">M9 + sucrose + TPA (1,2mM)</li> | ||
− | </ | + | </ol> |
<li style="text-align:left">Media 11 to 15 are made in both solid and liquid form:</li> | <li style="text-align:left">Media 11 to 15 are made in both solid and liquid form:</li> | ||
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<p style="text-align:left"> </p>--> | <p style="text-align:left"> </p>--> | ||
− | <h3><em>Enzymatic Assay | + | <h3><em>Enzymatic Assay of NB-Esterase</em></h3><br> |
<ol> | <ol> | ||
<li style="text-align:left">XbaI ans PstI enzymatic digest of the Biobrick BBa_K808030 and pDG011 plasmid.</li> | <li style="text-align:left">XbaI ans PstI enzymatic digest of the Biobrick BBa_K808030 and pDG011 plasmid.</li> |
Revision as of 12:44, 18 September 2015
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Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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