Difference between revisions of "Team:Pasteur Paris/Week 10"
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<center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> | <center><h3><em>Enzymatic Assay of NB-Esterase</em></h3></center><br> | ||
<ol> | <ol> | ||
− | <li style="text-align:left">XbaI ans PstI enzymatic digestion of the Biobrick BBa_K808030 and pDG011 plasmid.</li> | + | <li style="text-align:left">XbaI ans PstI enzymatic digestion of the Biobrick BBa_K808030 and pDG011 plasmid.</li></br> |
− | <li style="text-align:left">Gel purification of the digested fragments on 0.8% agarose gel. </li> | + | <li style="text-align:left">Gel purification of the digested fragments on 0.8% agarose gel. </li></br> |
− | <li style="text-align:left">Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase. </li> | + | <li style="text-align:left">Ligation of pDG011 plasmid and BBa_K808030 using T4 DNA ligase. </li></br> |
<li style="text-align:left">Gel migration on 0.8% agarose gel. </li></br> | <li style="text-align:left">Gel migration on 0.8% agarose gel. </li></br> | ||
</ol> | </ol> |
Revision as of 15:02, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-Esterase
Figure 8: Gel for verification of the ligation of BBa_K808030 and pDG011 plasmid. There is no band for the ligation sample. Gibson Assembly
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