Difference between revisions of "Team:Pasteur Paris/Week 14"
Line 111: | Line 111: | ||
<li>Xba I and Pst I enzymatic digestion. </li> | <li>Xba I and Pst I enzymatic digestion. </li> | ||
<li>Gel migration on 1% agarose gel. </li> | <li>Gel migration on 1% agarose gel. </li> | ||
− | <li>Gel Extraction following the Macherey-Nagel | + | <li>Gel Extraction following the Macherey-Nagel protocol </li> |
<li>DNA Concentration </li> | <li>DNA Concentration </li> | ||
</ul> | </ul> | ||
Line 117: | Line 117: | ||
<table border="1" cellspacing="0" cellpadding="0" width="604"> | <table border="1" cellspacing="0" cellpadding="0" width="604"> | ||
<tr> | <tr> | ||
− | <td width=" | + | <td width="110" valign="top"><p><b>Dilution 1/25</b></p></td> |
− | <td width=" | + | <td width="130" valign="top"><p><b>DNA Concentration (ng/µl)</b></p></td> |
− | <td width=" | + | <td width="110" valign="top"><p><b>OD(230nm</b>)</p></td> |
− | <td width=" | + | <td width="110" valign="top"><p><b>OD(260nm)</b></p></td> |
− | <td width=" | + | <td width="110" valign="top"><p><b>OD(280nm)</b></p></td> |
− | <td width=" | + | <td width="110" valign="top"><p><b>OD(260nm)/OD(230nm)</b></p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 145: | Line 145: | ||
<li>Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> | <li>Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> | ||
<li>Gel migration on 1% agarose gel. </li> | <li>Gel migration on 1% agarose gel. </li> | ||
− | <li>Gel Purification following the Macherey-Nagel | + | <li>Gel Purification following the Macherey-Nagel protocol </li> |
<li>DNA concentration assay using a spectrophotometer. </li> | <li>DNA concentration assay using a spectrophotometer. </li> | ||
</ul> | </ul> |
Revision as of 17:27, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 14 1/ After 72h of incubation, only contamination was observed. 2/ Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER. 3/ After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes. Assembly of Cluster 1 :
Assembly of cluster 2 :
^ |