Difference between revisions of "Team:Pasteur Paris/Week 14"

Line 126: Line 126:
 
   <tr>
 
   <tr>
 
     <td width="120" valign="top"><p><b>BBa_K808030</b></p></td>
 
     <td width="120" valign="top"><p><b>BBa_K808030</b></p></td>
−
     <td width="173" valign="top"><p align="right">0,2 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>0.200</center></p></td>
−
     <td width="120" valign="top"><p align="right">0,674 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.674</center></p></td>
−
     <td width="120" valign="top"><p align="right">0,04 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.040</center> </p></td>
−
     <td width="120" valign="top"><p align="right">0,00 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.000</center> </p></td>
−
     <td width="173" valign="top"><p align="right">0,01 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>0.010</center> </p></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td width="120" valign="top"><p><b>BBa_K808031</b></p></td>
 
     <td width="120" valign="top"><p><b>BBa_K808031</b></p></td>
−
     <td width="173" valign="top"><p align="right">0,2 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>0.200</center> </p></td>
−
     <td width="120" valign="top"><p align="right">0,560 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.560</center> </p></td>
−
     <td width="120" valign="top"><p align="right">0,003 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.003</center> </p></td>
−
     <td width="120" valign="top"><p align="right">0 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.000</center> </p></td>
−
     <td width="173" valign="top"><p align="right">0,01 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>0.010</center> </p></td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
Line 160: Line 160:
 
   <tr>
 
   <tr>
 
     <td width="120" valign="top"><p><b>BBa_K808007</b></p></td>
 
     <td width="120" valign="top"><p><b>BBa_K808007</b></p></td>
−
     <td width="173" valign="top"><p align="right">0,8 </p></td>
+
     <td width="173" valign="top"><p align="right">0.800 </center></p></td>
−
     <td width="120" valign="top"><p align="right">0,07 </p></td>
+
     <td width="120" valign="top"><p align="right">0.070</center> </p></td>
−
     <td width="120" valign="top"><p align="right">0,017 </p></td>
+
     <td width="120" valign="top"><p align="right">0.017 </center></p></td>
−
     <td width="120" valign="top"><p align="right">0,014 </p></td>
+
     <td width="120" valign="top"><p align="right">0.014 </center></p></td>
−
     <td width="173" valign="top"><p align="right">0,24 </p></td>
+
     <td width="173" valign="top"><p align="right">0.240 </center></p></td>
−
     <td width="173" valign="top"><p align="right">1,25 </p></td>
+
     <td width="173" valign="top"><p align="right">1.250</center> </p></td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td width="120" valign="top"><p><b>BBa_K808007</b></p></td>
 
     <td width="120" valign="top"><p><b>BBa_K808007</b></p></td>
−
     <td width="173" valign="top"><p align="right">1,1 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>1,100 </center></p></td>
−
     <td width="120" valign="top"><p align="right">0,133 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0,133 </center></p></td>
−
     <td width="120" valign="top"><p align="right">0,022 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.022 </center></p></td>
−
     <td width="120" valign="top"><p align="right">0,017 </p></td>
+
     <td width="120" valign="top"><p align="right"><center>0.017</center> </p></td>
−
     <td width="173" valign="top"><p align="right">0,17 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>0.170 </center></p></td>
−
     <td width="173" valign="top"><p align="right">1,28 </p></td>
+
     <td width="173" valign="top"><p align="right"><center>1.280</center> </p></td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>

Revision as of 17:42, 18 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 14

08/31 - 09/04




1. After 72h of incubation, only contamination was observed.

2. Co-transfection in yeast of nicked pRS415 nicked and Cre with extensions the protocol given by Heloise MÜLLER.

3. After Four days of culture of the electroporated yeast BY4742 with Cre+ homologous extension and the plasmid pRS415 nicked as vector no colonies has grown on Petri dishes.


Assembly of Cluster 1 :

  • Xba I and Pst I enzymatic digestion.
  • Gel migration on 1% agarose gel.
  • Gel Extraction following the Macherey-Nagel protocol
  • DNA Concentration

Dilution 1/25

DNA Concentration (ng/µl)

OD (230 nm)

OD (260 nm)

OD (280 nm)

OD (260 nm) / OD (230 nm)

BBa_K808030

0.200

0.674

0.040

0.000

0.010

BBa_K808031

0.200

0.560

0.003

0.000

0.010
  • Midiprep of BBa_K808030
  • Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step.
  • Gel migration on 1% agarose gel.
  • Gel Purification following the Macherey-Nagel protocol
  • DNA concentration assay using a spectrophotometer.

Dilution 1/25

DNA Concentration (ng/µl)

OD (230 nm)

OD (260 nm)

OD (280 nm)

OD (260 nm) / OD (230 nm)

OD (260 nm) / OD (280 nm)

BBa_K808007

0.800

0.070

0.017

0.014

0.240

1.250

BBa_K808007

1,100

0,133

0.022

0.017

0.170

1.280
  • Ligation of BBa_K808007 and BBa_K808030
  • Transformation in electro-competent DH5-⍺ cells.
  • Preparation for sequencing.

Assembly of cluster 2 :

  • PCR amplification of BBa_K936011 and BBa_K936023 using Q5 High fidelity Master Mix.

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