Difference between revisions of "Team:Pasteur Paris/Week 14"
Line 109: | Line 109: | ||
<li style="text-align:left">Assembly of cluster 1:</br> | <li style="text-align:left">Assembly of cluster 1:</br> | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
− | <li>Xba I and Pst I enzymatic digestion. </li> | + | <li style="text-align:left">Xba I and Pst I enzymatic digestion. </li> |
− | <li>Gel migration on 1% agarose gel. </li> | + | <li style="text-align:left">Gel migration on 1% agarose gel. </li> |
− | <li>Gel Extraction following the Macherey-Nagel protocol </li> | + | <li style="text-align:left">Gel Extraction following the Macherey-Nagel protocol </li> |
− | <li>DNA Concentration </li> | + | <li style="text-align:left">DNA Concentration </li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 145: | Line 145: | ||
<li style="text-align:left">Midiprep of BBa_K808030: | <li style="text-align:left">Midiprep of BBa_K808030: | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
− | <li>Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> | + | <li style="text-align:left">Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> |
− | <li>Gel migration on 1% agarose gel. </li> | + | <li style="text-align:left">Gel migration on 1% agarose gel. </li> |
− | <li>Gel Purification following the Macherey-Nagel protocol </li> | + | <li style="text-align:left">Gel Purification following the Macherey-Nagel protocol </li> |
− | <li>DNA concentration assay using a spectrophotometer. </li> | + | <li style="text-align:left">DNA concentration assay using a spectrophotometer. </li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 182: | Line 182: | ||
</table> | </table> | ||
</br> | </br> | ||
− | <li>Ligation of BBa_K808007 and BBa_K808030 </li> | + | <li style="text-align:left">Ligation of BBa_K808007 and BBa_K808030 </li> |
− | <li>Transformation in electro-competent DH5-⍺ cells. </li> | + | <li style="text-align:left">Transformation in electro-competent DH5-⍺ cells. </li> |
− | <li>Preparation for sequencing.</li> | + | <li style="text-align:left">Preparation for sequencing.</li> |
<br> | <br> | ||
<li style="text-align:left">Assembly of cluster 2:</li> | <li style="text-align:left">Assembly of cluster 2:</li> | ||
− | <li>PCR amplification of BBa_K936011 and BBa_K936023 using Q5 High fidelity Master Mix. </li> | + | <li style="text-align:left">PCR amplification of BBa_K936011 and BBa_K936023 using Q5 High fidelity Master Mix. </li> |
</ol> | </ol> | ||
Revision as of 18:06, 18 September 2015
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Week 14
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