Difference between revisions of "Team:Pasteur Paris/Week 15"

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<center><img src="https://static.igem.org/mediawiki/2015/6/69/IGEM-Pasteur_Gel-week-15.jpg" />
 
<center><img src="https://static.igem.org/mediawiki/2015/6/69/IGEM-Pasteur_Gel-week-15.jpg" />
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<p><u><b>Figure xx:</u></b> Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on a 1% agarose Gel. </p></center></br>
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<p><u><b>Figure xx:</u></b> Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose cel. </p></center></br>
  
  
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<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on a 1% agarose Gel.</br>
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<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose cel.</br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4 :  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4 :  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
 
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells </li></br>
 
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells </li></br>
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   <li style="text-align:left">MiniPrep </li></br>
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   <li style="text-align:left">MiniPrep</li></br>
 
   <li style="text-align:left">Preparation for sequencing. </li>
 
   <li style="text-align:left">Preparation for sequencing. </li>
 
</ul>
 
</ul>
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<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931 : </p>
 
<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931 : </p>
 
<ul style="text-align: left">
 
<ul style="text-align: left">
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   <li>Transformation in Chemically  competent <em>E.coli </em>DH5- cells. </li>
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   <li>Transformation in chemically competent <em>E. coli</em> DH5- cells. </li>
 
</ul>
 
</ul>
  

Revision as of 21:24, 18 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 15

09/07 - 09/11




  1. Gibson Assembly of Cluster 4

    2. Figure xx: Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose cel.


  2. Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose cel.
  3. Gibson Assembly of cluster 4 : BBa_K936011, BBa_K936023 and pSB1C3.

  4. Transformation in chemically competent E. coli DH5-⍺ cells

  5. MiniPrep

  6. Preparation for sequencing.

    7. Stock of the plasmids containing BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K1392931 :

    • Transformation in chemically competent E. coli DH5- cells.


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