Difference between revisions of "Team:Pasteur Paris/Week 15"

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<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931 : </p>
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<p style="text-align: left; padding: 30px;">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931: </p>
 
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   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells. </li>
 
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-⍺ cells. </li>

Revision as of 21:48, 18 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 15

09/07 - 09/11




  • Gibson Assembly of Cluster 4

    • Figure xx: Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose cel.


    1. Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose gel.
    2. Gibson Assembly of cluster 4: BBa_K936011, BBa_K936023 and pSB1C3.

    3. Transformation in chemically competent E. coli DH5-⍺ cells

    4. MiniPrep

    5. Preparation for sequencing.

    Stock of the plasmids containing BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K1392931:

    1. Transformation in chemically competent E. coli DH5-⍺ cells.


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