Difference between revisions of "Team:Pasteur Paris/Week 15"

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<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose gel.</br>
 
<li style="text-align:left">Gel migration of the PCR  products of BBa_K936011 and BBa_K936023 on 1% agarose gel.</br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4:  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
 
   <li style="text-align:left">Gibson Assembly of cluster 4:  BBa_K936011, BBa_K936023 and pSB1C3. </li></br>
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-cells </li></br>
+
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-α cells </li></br>
 
   <li style="text-align:left">MiniPrep</li></br>
 
   <li style="text-align:left">MiniPrep</li></br>
 
   <li style="text-align:left">Preparation for sequencing. </li>
 
   <li style="text-align:left">Preparation for sequencing. </li>
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<li style="text-align:left">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931: </li>
 
<li style="text-align:left">Stock of the plasmids containing BBa_K936011,  BBa_K936023, BBa_K1392932, BBa_K1392931: </li>
 
<ol>
 
<ol>
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-cells. </li>
+
   <li style="text-align:left">Transformation in chemically competent <em>E. coli</em> DH5-α cells. </li>
 
</ol>
 
</ol>
 
</ul>
 
</ul>

Revision as of 21:53, 18 September 2015

Week 15

09/07 - 09/11




  • Gibson Assembly of Cluster 4

  • Figure xx: Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose cel.


    1. Gel migration of the PCR products of BBa_K936011 and BBa_K936023 on 1% agarose gel.
    2. Gibson Assembly of cluster 4: BBa_K936011, BBa_K936023 and pSB1C3.

    3. Transformation in chemically competent E. coli DH5-α cells

    4. MiniPrep

    5. Preparation for sequencing.

  • Stock of the plasmids containing BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K1392931:
    1. Transformation in chemically competent E. coli DH5-α cells.


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