Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/3 August 2015"

(→‎Analyzing SDS PAGE Gel results)
(→‎Analyzing SDS PAGE Gel results)
Line 289: Line 289:
 
*Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
 
*Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
 
**The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
 
**The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
−
*Here is the gel [[File:UCLA Bl21honeybee.jpg|none|thumb|900px|'''Fig. 1''' Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder.]]
+
*Here is the gel [[File:UCLA Bl21honeybee.jpg|none|thumb|900px|'''Fig. 1''' Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder. From lane 1-9 F1,S1,F2,F3,S2,S3, Final product, BSA positive control, and ladder (see table)]]
 +
*Refer to [https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk/29_July_2015#Honeybee_Inclusion_Body_Purification 7/29] for more detailed description of when each fraction was taken.

Revision as of 18:23, 3 August 2015

Analyzing SDS PAGE Gel results

  • Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
    • The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
  • Here is the gel
    Fig. 1 Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder. From lane 1-9 F1,S1,F2,F3,S2,S3, Final product, BSA positive control, and ladder (see table)
  • Refer to 7/29 for more detailed description of when each fraction was taken.