Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/3 August 2015"
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*Here is the gel [[File:UCLA Bl21honeybee.jpg|none|thumb|900px|'''Fig. 1''' Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder. From lane 1-9 F1,S1,F2,F3,S2,S3, Final product, positive control, and ladder (see table)]] | *Here is the gel [[File:UCLA Bl21honeybee.jpg|none|thumb|900px|'''Fig. 1''' Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder. From lane 1-9 F1,S1,F2,F3,S2,S3, Final product, positive control, and ladder (see table)]] | ||
*Refer to [https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk/29_July_2015#Honeybee_Inclusion_Body_Purification 7/29] for more detailed description of when each fraction was taken. | *Refer to [https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk/29_July_2015#Honeybee_Inclusion_Body_Purification 7/29] for more detailed description of when each fraction was taken. | ||
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Revision as of 18:40, 3 August 2015
Analyzing SDS PAGE Gel results
- Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
- The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
- Here is the gel
- Refer to 7/29 for more detailed description of when each fraction was taken.
F1 | Full cell lysate using BugBuster lysis system |
---|---|
S1 | Supernatant after spinning down full cell lysate |
F2 | Full cell lysate #2 after resuspending pellet in Bugbuster |
F3 | Full cell lysate #3 after adding lysozyme and DNase |
S2 | Supernatant after first wash step. |
S3 | Supernatant after second wash step |
Final product | Purified inclusion body in SDS |
positive control | BSA positive control |
ladder | BioRad dual color ladder |