Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/3 August 2015"
(→Analyzing SDS PAGE Gel results) |
(→Analyzing SDS PAGE Gel results) |
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Line 294: | Line 294: | ||
|+ | |+ | ||
|- | |- | ||
− | ! F1 | + | ! F1 (lane 1) |
! Full cell lysate using BugBuster lysis system | ! Full cell lysate using BugBuster lysis system | ||
|- | |- | ||
− | ! S1 | + | ! S1 (lane 2) |
| Supernatant after spinning down full cell lysate | | Supernatant after spinning down full cell lysate | ||
|- | |- | ||
− | ! F2 | + | ! F2 (lane 3) |
| Full cell lysate #2 after resuspending pellet in Bugbuster | | Full cell lysate #2 after resuspending pellet in Bugbuster | ||
|- | |- | ||
− | ! F3 | + | ! F3 (lane 4) |
| Full cell lysate #3 after adding lysozyme and DNase | | Full cell lysate #3 after adding lysozyme and DNase | ||
|- | |- | ||
− | ! S2 | + | ! S2 (lane 5) |
| Supernatant after first wash step. | | Supernatant after first wash step. | ||
|- | |- | ||
− | !S3 | + | !S3 (lane 6) |
|Supernatant after second wash step | |Supernatant after second wash step | ||
|- | |- | ||
− | ! Final product | + | ! Final product (lane 7) |
| Purified inclusion body in SDS | | Purified inclusion body in SDS | ||
|- | |- | ||
− | ! positive control | + | ! positive control (lane 8) |
| BSA positive control | | BSA positive control | ||
|- | |- | ||
− | !ladder | + | !ladder (lane 9) |
|BioRad dual color ladder | |BioRad dual color ladder | ||
|} | |} |
Revision as of 18:42, 3 August 2015
Analyzing SDS PAGE Gel results
- Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
- The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
- Here is the gel
- Refer to 7/29 for more detailed description of when each fraction was taken.
F1 (lane 1) | Full cell lysate using BugBuster lysis system |
---|---|
S1 (lane 2) | Supernatant after spinning down full cell lysate |
F2 (lane 3) | Full cell lysate #2 after resuspending pellet in Bugbuster |
F3 (lane 4) | Full cell lysate #3 after adding lysozyme and DNase |
S2 (lane 5) | Supernatant after first wash step. |
S3 (lane 6) | Supernatant after second wash step |
Final product (lane 7) | Purified inclusion body in SDS |
positive control (lane 8) | BSA positive control |
ladder (lane 9) | BioRad dual color ladder |