Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/5 August 2015"
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*Corresponds to a 17.2 w/v% for the 7/29 silk and 129 mg total for the 100 ml culture | *Corresponds to a 17.2 w/v% for the 7/29 silk and 129 mg total for the 100 ml culture | ||
*Once again, keep in mind that this is not necessarily all honeybee silk, and could be cellular debris and other contaminating proteins. | *Once again, keep in mind that this is not necessarily all honeybee silk, and could be cellular debris and other contaminating proteins. | ||
| + | ===Starting 300ml Culture of Honeybee Silk=== | ||
| + | *300 ml LB and 300 ul KAN, with one colony. | ||
| + | *Inoculated at noon | ||
| + | *At 4PM the OD was 0.06 | ||
| + | *At 5:40 PM the OD was 0.56 | ||
| + | **At that point added 150 ul of 1M IPTG (0.5mM) | ||
Revision as of 02:42, 6 August 2015
8/5
- Analyzing dry film test.
- Weight of 100 ul SDS solution after drying = 3.3mg
- 5/26 Silk = 3.5 mg (subtracting the 3.3 mg of SDS = 0.2 mg
- 7/29 silk = 20.5 mg or 17.2 mg after controlling for SDS.
- This corresponds to 0.2 weight by volume percent for the 5/26 Silk and 4 mg total for the 250 ml culture.
- Corresponds to a 17.2 w/v% for the 7/29 silk and 129 mg total for the 100 ml culture
- Once again, keep in mind that this is not necessarily all honeybee silk, and could be cellular debris and other contaminating proteins.
Starting 300ml Culture of Honeybee Silk
- 300 ml LB and 300 ul KAN, with one colony.
- Inoculated at noon
- At 4PM the OD was 0.06
- At 5:40 PM the OD was 0.56
- At that point added 150 ul of 1M IPTG (0.5mM)