Team:Vilnius-Lithuania/Labjournal
About
pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.
| Date | Materials | Experiment |
|---|---|---|
| 06 30 | P10 P11 |
P10 and P11 transformation into DH5α. |
| 07 01 | Bacteria inoculation (4 minipreps). | |
| 07 09 | P10 P11 |
Plasmids’ restriction (3 ug each). P10 with EcoRI and XBal (+FastAp). P11 with EcoRI and SpeI. Extraction from gel L10 and L11 are produced. Conc.: L11 – 2,4 ng/uL. |
Conclusions and Results:
07 14 – all of the colonies are successful, except third colony.