Team:UCLA/Notebook/Honeybee Silk/26 May 2015

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BugBuster Protocol

• Using the Bugbuster 10X cell lysis reagent in order to purify our honeybee silk protein via inclusion bodies.
  • Based protocol off of the Novagen manufacturer's protocol which can be found [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf here].
• Expressing honey bee silk in E. Coli, from [http://parts.igem.org/Part:BBa_K1763001 BBa_K1763001]
  • See 5/18 and [https://2015.igem.org/Team:UCLA/Notebook/Honeybee_Silk/19_May_2015 5/19 for details on the protein expression.

Step by Step Protocol