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Week 4
06/22 - 06/26
- MiniPrep of BBa_808026 following the QIAgen MiniPrep protocol.
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
[DNA] C (ng/µL) |
21,5 |
33,8 |
26,7 |
DNA (250ng) Volume |
11,6 |
7,4 |
9,4 |
Mix enzymatique Volume |
1 |
1 |
1 |
Tampon 10X Volume |
5 |
5 |
5 |
Water Volume |
32,4 |
36,6 |
34,6 |
Total |
50 |
50 |
50 |
Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.
- OD measurement using a Spectrophotometer
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
Concentration (ng/µL) |
21,5 |
33,8 |
26,7 |
OD(260 nm) |
0,430 |
0,665 |
0,534 |
OD(260 nm)/OD (280nm) |
1,69 |
1,81 |
1,77 |
OD(260 nm)/OD (230nm) |
2,64 |
1,56 |
1,86 |
- Gel migration of the BioBrick K808026 on a 0,8% Agarose Gel. (1h at 130V)
Figure 7: Results of the Gel Migration (BBa_808026).
- Bacterial culture of BBa_K1095000 on a Petri dish LB+Cm34.
- Plasmid purification of BBa_808026 following the MidiPrep and MiniPrep protocol.
BBa_K808026 |
Clone 1 |
Clone 2 |
Clone 3 |
[DNA] C (ng/µL) |
21,5 |
33,8 |
26,7 |
DNA (250ng) Volume |
11,6 |
7,4 |
9,4 |
Mix enzymatique Volume |
1 |
1 |
1 |
Tampon 10X Volume |
5 |
5 |
5 |
Water Volume |
32,4 |
36,6 |
34,6 |
Total |
50 |
50 |
50 |
Figure 6: DNA Extraction by using the QiAprep spin protocol for a bioassay and digest.
- Transformation of plasmids TeV and pDG022 in DH5-⍺ chemically competent cells.
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