Human Practices
Some sort of subheading.
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GRAS
To date, no genetically engineered starter cultures have been approved for use in the USA by the FDA, and specific criteria for approval has yet to be established [1, 2]. This lack of approval is commonly attributed to a lack of consumer knowledge and understanding [2]. Because of the countless foreseeable benefits using genetically engineered starter cultures and the amount of increased potential profits in industrial cheese production, scientist continue research into the area and remain optimistic for future approval [2]. Generally recognized as safe (GRAS) is a status given by the USDA for allowable food additives. L. lactis is a GRAS organism, having FDA approval for food-grade applications [3]. Because the promoters and all subsequent genetic elements incorporated within this project are of lactococcal origin, it is a reasonable assumption that they should rationally meet the requirements of being considered GRAS, making this a promising model for P335 lytic phage detection and defense in future approved genetically engineered starter cultures [3].
GRAS References:
- Hansen EB (2002) Commercial bacterial starter cultures for fermented foods of the future. Int J Food Microbiol 78:119–31. doi: 10.1016/S0168-1605(02)00238-6
- Soccol CR, Pandey A, Larroche C (2013) Fermentation Processes Engineering in the Food Industry. CRC Press
- Djordjevic GM, O’Sullivan DJ, Walker SA, et al. (1997) A triggered-suicide system designed as a defense against bacteriophages. J Bacteriol 179:6741–6748.
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