Week 10
08/03 - 08/07
Toxicity and solubility of TPA (Terephthalic acid)
- Dissolution of TPA in DMSO (Dimethyl sulfoxide). The best concentration obtained is 62 mg/l
- Dilution of TPA stock in LB Broth => TPA precipitates at a concentration of 3.9 g/l, maximum concentration in LB Broth is 0.9 mg/l
- Solubilization of TPA by NaOH
- Creation of a stock solution of soluble TPA in water at 0,5mol/L
- Test of the TPA Solubilization in LB Broth
- Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:Media 1 to 10 are made in solid form:
- M9
- M9 + TPA (1.2mM)
- M9 + TPA (6mM)
- M9 + TPA (12mM)
- M9 + sucrose (1.2mM)
- M9 + sucrose (6mM)
- M9 + sucrose (12mM)
- M9 + sucrose + TPA (6mM)
- M9 + sucrose + TPA (1,2mM)
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