Team:UCLA/Notebook/Materials/9 July 2015

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Michael's Part

• Continuation of yesterday's dialysis
  • Dialysis STOP: 12:30 PM, July 9
  • extracted about 750 uL of double dialyzed silk.
  • aliquoted 20 uL of concentrated dope, allowed to dry at 60 C
  • weight of empty weigh boat: 0.596 grams
  • weight of weigh boat + dry silk: 0.600 grams
  • calculated w/v%: 20% w/v.

• Received a fraction of Tamura from Fasih and Jessica
• We need to dilute down the silk concentrate before spinning. I do not know the concentration of the Tamura, so I will be assuming that the Tamura is dilute enough to treat as water in the calculations. That is to say, instead of diluting the silk dope with water, I will be diluting it with the Tamura fraction.

• Let's spin 250 uL of the Tamura.
• To obtain 250 uL of "15% w/v silk," we need 187.5uL of the 20% w/v silk dope, and 62.5 uL of the Tamura fraction.

• However, before adding the Tamura fraction, we need to ensure that there are no particulates
  • Currently, the fraction has a lot of particulates -- probably some coagulated silk, as well as some of the resin.
  • Aliquot 500 uL of the fraction into a tube. Centrifuge at 5000 RPM for 10 minutes at 4 C.
  • Transfer supernatant to a syringe with a filter tip, then inject into a new tube.

• Some problems
  • Upon centrifugation, both the resin and the silk particles settle at the tube bottom (checked under UV box)
  • I aliquoted another 250 uL of the fraction to syringe filter, all of the silk particles were trapped in the filter (checked under UV box).

• some things we can try:
  • add more solvent to try to solubilize the Tamura particulates

• I took 2 mL of "Fraction 4" from the Functionalization team
  • This solution is much more viscous, has much less particulates, and has comparable fluorescent intensity when checked under the UV box.

• I will take Fraction 4 and mix it with the silk dope.
  • Considering how viscous both the silk dope and the Tamura fraction are, I should probably add some water to the mix
  • Will add

Spinning Apparatus Project

• Gathered all required Materials:
  • 2 12V Stepper Motors
  • 1 Arduino Uno R3(knockoff brand)
  • Breadboards
  • 2 Easy Driver v4.4