Team:UCLA/Notebook/Honeybee Silk/14 July 2015

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Miniprep

Samples 1 and 3 (inoculated) were miniprepped using the Zymo Plasmid Miniprep Kit.

OD

1. 128.23 ng/uL
2. 106.85 ng/uL

Both samples were sent in for sequencing.

Calculating Competency

• Calculating competency of cells prepared on 7/12.
• Equation = # colonies / ug DNA plated
• Using 2 ul of 50pg/ul puc19 vector to transform in to 50 ul
• Counted 300 colonies
• plated 1x10^-5 ug (1/10 of tranformation reacction).
• Therefore tranformation efficiency = 3 x 10^7 cfu/ug DNA